VPAC1 targeted 64Cu-TP3805 positron emission tomography imaging of prostate cancer: Preliminary evaluation in man

Sushil Tripathi, Edouard J. Trabulsi, Leonard Gomella, Sung Kim, Peter McCue, Charles Intenzo, Ruth Birbe, Ashish Gandhe, Pardeep Kumar, Mathew Thakur

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Objective To evaluate 64Cu-TP3805 as a novel biomolecule, to positron emission tomography (PET) image prostate cancer (PC), at the onset of which VPAC1, the superfamily of G protein-coupled receptors, is expressed in high density on PC cells, but not on normal cells. Materials and Methods Twenty-five patients undergoing radical prostatectomy were PET/X-ray computerized tomography imaged preoperatively with 64Cu-TP3805. Standardized maximum uptake (SUVmax) values were determined and malignant lesions (standardized uptake value > 1.0) counted, and compared with histologic findings. Whole-mount pathology slides from 6 VPAC1 PET imaged patients, 3 benign prostatic hyperplasia patients, 1 malignant and 1 benign lymph node underwent digital autoradiography (DAR) after 64Cu-TP3805 incubation and were compared to hematoxylin- and eosin-stained slides. Results In 25 patients who underwent PET imaging, 212 prostate gland lesions had SUVmax > 1.0 vs 127 lesions identified by histology of biopsy tissues. The status of the additional 85 PET identified prostate lesions remains to be determined. In 68 histologic slides from 6 PET imaged patients, DAR identified 105 of 107 PC foci, 19 of 19 high-grade prostatic intraepithelial neoplasias, and ejaculatory ducts and verumontanum involved with cancer. Additionally, DAR found 9 PC lesions not previously identified histologically. The positive and negative lymph nodes were correctly identified, and in 3 of 3 benign prostatic hyperplasia patients and 5 of 5 cysts, DAR was negative. Conclusion This feasibility study demonstrated that 64Cu-TP3805 delineates PC in vivo and ex vivo, provided normal images for benign masses, and is worthy of further studies.

Original languageEnglish
Pages (from-to)111-118
Number of pages8
JournalUrology
Volume88
DOIs
StatePublished - Feb 1 2016

Keywords

  • Adult
  • Aged
  • Coordination Complexes
  • Feasibility Studies
  • Humans
  • Male
  • Middle Aged
  • Peptides
  • Positron-Emission Tomography
  • Prostatic Hyperplasia
  • Prostatic Neoplasms/diagnostic imaging
  • Receptors, Vasoactive Intestinal Polypeptide, Type I/biosynthesis

Fingerprint

Dive into the research topics of 'VPAC1 targeted 64Cu-TP3805 positron emission tomography imaging of prostate cancer: Preliminary evaluation in man'. Together they form a unique fingerprint.

Cite this