TY - JOUR
T1 - Variation amongst K562 cell cultures
AU - Dimery, I. W.
AU - Ross, D. D.
AU - Testa, J. R.
AU - Gupta, S. K.
AU - Felsted, R. L.
AU - Pollak, A.
AU - Bachur, N. R.
PY - 1983
Y1 - 1983
N2 - K562 cell cultures were obtained from three laboratories (A, B and C) outside of our institution, and were designated according to source as K562A, K562B or K562C. The cultures obtained were constitutive or 'wild type' K562 cell cultures, not cloned sublines. These cell cultures were compared with respect to growth kinetics, cell surface protein markers, surface antigens, cytogenetics and hemoglobin production. Morphology, growth kinetics in liquid suspension culture, cloning efficiency in soft agar culture, binding of anti-K562 monoclonal antibodies, and the majority of cell surface proteins were generally similar. In contrast, several important differences were observed: (1) hemoglobin synthesis induced by hemin was significantly different among K562A, B and C, K562A being most sensitive (P < 0.05); (2) whereas more than 90% of K562A or C cells appeared to be Philadelphia chromosome (PH
1)-positive, less than 15% of K562B cells contained a Ph
1; (3) membrane proteins (93 and 85 kilodalton) were identified in K562A, whereas only the 93 kilodalton protein was detected in K562B and neither of the proteins were detected in K562C. Our results indicate that K562 cells maintained in different laboratories can undergo tangible changes which may influence experimental results obtained in studies using these cells.
AB - K562 cell cultures were obtained from three laboratories (A, B and C) outside of our institution, and were designated according to source as K562A, K562B or K562C. The cultures obtained were constitutive or 'wild type' K562 cell cultures, not cloned sublines. These cell cultures were compared with respect to growth kinetics, cell surface protein markers, surface antigens, cytogenetics and hemoglobin production. Morphology, growth kinetics in liquid suspension culture, cloning efficiency in soft agar culture, binding of anti-K562 monoclonal antibodies, and the majority of cell surface proteins were generally similar. In contrast, several important differences were observed: (1) hemoglobin synthesis induced by hemin was significantly different among K562A, B and C, K562A being most sensitive (P < 0.05); (2) whereas more than 90% of K562A or C cells appeared to be Philadelphia chromosome (PH
1)-positive, less than 15% of K562B cells contained a Ph
1; (3) membrane proteins (93 and 85 kilodalton) were identified in K562A, whereas only the 93 kilodalton protein was detected in K562B and neither of the proteins were detected in K562C. Our results indicate that K562 cells maintained in different laboratories can undergo tangible changes which may influence experimental results obtained in studies using these cells.
KW - Autoradiography
KW - Binding Sites, Antibody
KW - Cell Line
KW - Cell Transformation, Neoplastic/analysis
KW - Chromosome Banding
KW - Clone Cells/pathology
KW - Hemoglobins/biosynthesis
KW - Humans
KW - Isoenzymes
KW - L-Lactate Dehydrogenase/metabolism
KW - Leukemia, Myeloid/genetics
KW - Membrane Proteins/analysis
KW - Phenotype
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M3 - Article
C2 - 6576909
SN - 0301-472X
VL - 11
SP - 601
EP - 610
JO - Experimental Hematology
JF - Experimental Hematology
IS - 7
ER -