TY - JOUR
T1 - uPARAP/Endo180 is essential for cellular uptake of collagen and promotes fibroblast collagen adhesion
AU - Engelholm, Lars H.
AU - List, Karin
AU - Netzel-Arnett, Sarah
AU - Cukierman, Edna
AU - Mitola, David J.
AU - Aaronson, Hannah
AU - Kjøller, Lars
AU - Larsen, Jørgen K.
AU - Yamada, Kenneth M.
AU - Strickland, Dudley K.
AU - Holmbeck, Kenn
AU - Danø, Keld
AU - Birkedal-Hansen, Henning
AU - Behrendt, Niels
AU - Bugge, Thomas H.
PY - 2003/4/3
Y1 - 2003/4/3
N2 - The uptake and lysosomal degradation of collagen by fibroblasts constitute a major pathway in the turn-over of connective tissue. However, the molecular mechanisms governing this pathway are poorly understood. Here, we show that the urokinase plasminogen activator receptor-associated protein (uPARAP)/Endo180, a novel mesenchymally expressed member of the macrophage mannose receptor family of endocytic receptors, is a key player in this process. Fibroblasts from mice with a targeted deletion in the uPARAP/Endo180 gene displayed a near to complete abrogation of collagen endocytosis. Furthermore, these cells had diminished initial adhesion to a range of different collagens, as well as impaired migration on fibrillar collagen. These studies identify a central function of uPARAP/Endo180 in cellular collagen interactions.
AB - The uptake and lysosomal degradation of collagen by fibroblasts constitute a major pathway in the turn-over of connective tissue. However, the molecular mechanisms governing this pathway are poorly understood. Here, we show that the urokinase plasminogen activator receptor-associated protein (uPARAP)/Endo180, a novel mesenchymally expressed member of the macrophage mannose receptor family of endocytic receptors, is a key player in this process. Fibroblasts from mice with a targeted deletion in the uPARAP/Endo180 gene displayed a near to complete abrogation of collagen endocytosis. Furthermore, these cells had diminished initial adhesion to a range of different collagens, as well as impaired migration on fibrillar collagen. These studies identify a central function of uPARAP/Endo180 in cellular collagen interactions.
KW - Cell adhesion
KW - Integrin
KW - Matrix internalization
KW - Matrix metalloproteinase
KW - uPAR
UR - http://www.scopus.com/inward/record.url?scp=0037417411&partnerID=8YFLogxK
U2 - 10.1083/jcb.200211091
DO - 10.1083/jcb.200211091
M3 - Article
SN - 0021-9525
VL - 160
SP - 1009
EP - 1015
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 7
ER -