TY - JOUR
T1 - Transformed Root Extract of Leonurus sibiricus Induces Apoptosis through Intrinsic and Extrinsic Pathways in Various Grades of Human Glioma Cells
AU - Sitarek, Przemysław
AU - Skała, Ewa
AU - Toma, Monika
AU - Wielanek, Marzena
AU - Szemraj, Janusz
AU - Skorski, Tomasz
AU - Białas, Adam J.
AU - Sakowicz, Tomasz
AU - Kowalczyk, Tomasz
AU - Radek, Maciej
AU - Wysokińska, Halina
AU - Śliwiński, Tomasz
N1 - Publisher Copyright:
© 2016, Arányi Lajos Foundation.
PY - 2017/7/1
Y1 - 2017/7/1
N2 - This study determines the influence of transformed root (TR) extract of Leonurus sibiricus L. on various grades (I-III) of human glioma cells derived from patients. This plant occurs in southern Asia and Siberia and is widely used as a medicinal plant with various biological activities. Chromatographic profile of TR extract have revealed the presence of various polyphenolic compounds (4-hydroxybenzoic acid, gentisic acid, vanilic acid, 1,3-dicaffeoylquinic acid, α-resorcylic acid). We found TR root extract to have antiproliferative activity on glioma cells after 24 h of treatment. TR root extract induces apoptosis on various grades (I-III) of human glioma cells by the generation of reactive oxygen species (ROS) along with concurrent loss of mitochondrial membrane potential, enhanced S and G2/M phases of the cell cycle, and altered mRNA levels of Bax, Bcl-2, p53, Cas-3, Cas-8 and Cas-9 factors involved in apoptosis. This work for the first time demonstrate that TR extract from L. sibiricus root has the potential to activate apoptosis in grade I-III human glioma cells through the intrinsic and extrinsic pathways.
AB - This study determines the influence of transformed root (TR) extract of Leonurus sibiricus L. on various grades (I-III) of human glioma cells derived from patients. This plant occurs in southern Asia and Siberia and is widely used as a medicinal plant with various biological activities. Chromatographic profile of TR extract have revealed the presence of various polyphenolic compounds (4-hydroxybenzoic acid, gentisic acid, vanilic acid, 1,3-dicaffeoylquinic acid, α-resorcylic acid). We found TR root extract to have antiproliferative activity on glioma cells after 24 h of treatment. TR root extract induces apoptosis on various grades (I-III) of human glioma cells by the generation of reactive oxygen species (ROS) along with concurrent loss of mitochondrial membrane potential, enhanced S and G2/M phases of the cell cycle, and altered mRNA levels of Bax, Bcl-2, p53, Cas-3, Cas-8 and Cas-9 factors involved in apoptosis. This work for the first time demonstrate that TR extract from L. sibiricus root has the potential to activate apoptosis in grade I-III human glioma cells through the intrinsic and extrinsic pathways.
KW - Apoptosis
KW - Cell cycle
KW - Gene expression
KW - Mitochondrial membrane potential
KW - Reactive oxygen species
KW - Transformed roots of Leonurus sibiricus
UR - http://www.scopus.com/inward/record.url?scp=85007507394&partnerID=8YFLogxK
UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=purepublist2023&SrcAuth=WosAPI&KeyUT=WOS:000402570200027&DestLinkType=FullRecord&DestApp=WOS
U2 - 10.1007/s12253-016-0170-6
DO - 10.1007/s12253-016-0170-6
M3 - Article
C2 - 28032310
SN - 1219-4956
VL - 23
SP - 679
EP - 687
JO - Pathology and Oncology Research
JF - Pathology and Oncology Research
IS - 3
ER -