Transformation of chicken cells by the gene encoding the catalytic subunit of PI 3-kinase

Hwai Wen Chang; Masahiro Aoki; David Fruman; Kurt R. Auger; Alfonso Bellacosa; Philip N. Tsichlis; Lewis C. Cantley; Thomas M. Roberts; Peter K. Vogt

doi:10.1126/science.276.5320.1848

Transformation of chicken cells by the gene encoding the catalytic subunit of PI 3-kinase

Hwai Wen Chang, Masahiro Aoki, David Fruman, Kurt R. Auger, Alfonso Bellacosa, Philip N. Tsichlis, Lewis C. Cantley, Thomas M. Roberts, Peter K. Vogt

Research output: Contribution to journal › Article › peer-review

396 Scopus citations

Abstract

The avian sarcoma virus 16 (ASV 16) is a retrovirus that induces hemangiosarcomas in chickens. Analysis of the ASV 16 genome revealed that it encodes an oncogene that is derived from the cellular gene for the catalytic subunit of phosphoinositide 3-kinase (PI 3-kinase). The gene is referred to as v-p3k, and like its cellular counterpart c-p3k, it is a potent transforming gene in cultured chicken embryo fibroblasts (CEFs). The products of the viral and cellular p3k genes have PI 3-kinase activity. CEFs transformed with either gene showed elevated levels of phosphatidylinositol 3,4-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate and activation of Akt kinase.

Original language	English
Pages (from-to)	1848-1850
Number of pages	3
Journal	Science
Volume	276
Issue number	5320
DOIs	https://doi.org/10.1126/science.276.5320.1848
State	Published - Jun 20 1997

Keywords

Amino Acid Sequence
Animals
Avian Sarcoma Viruses/genetics
Cell Transformation, Neoplastic
Cell Transformation, Viral
Cells, Cultured
Chick Embryo
Chickens
Cloning, Molecular
Enzyme Activation
Genes, Viral
Hemangiosarcoma/genetics
Molecular Sequence Data
Oncogenes
Phosphatidylinositol 3-Kinases
Phosphatidylinositol Phosphates/metabolism
Phosphotransferases (Alcohol Group Acceptor)/genetics
Platelet-Derived Growth Factor/pharmacology
Protein Serine-Threonine Kinases/metabolism
Proto-Oncogene Proteins c-akt
Proto-Oncogene Proteins/metabolism
Signal Transduction
Transfection

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title = "Transformation of chicken cells by the gene encoding the catalytic subunit of PI 3-kinase",

abstract = "The avian sarcoma virus 16 (ASV 16) is a retrovirus that induces hemangiosarcomas in chickens. Analysis of the ASV 16 genome revealed that it encodes an oncogene that is derived from the cellular gene for the catalytic subunit of phosphoinositide 3-kinase (PI 3-kinase). The gene is referred to as v-p3k, and like its cellular counterpart c-p3k, it is a potent transforming gene in cultured chicken embryo fibroblasts (CEFs). The products of the viral and cellular p3k genes have PI 3-kinase activity. CEFs transformed with either gene showed elevated levels of phosphatidylinositol 3,4-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate and activation of Akt kinase.",

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author = "Chang, {Hwai Wen} and Masahiro Aoki and David Fruman and Auger, {Kurt R.} and Alfonso Bellacosa and Tsichlis, {Philip N.} and Cantley, {Lewis C.} and Roberts, {Thomas M.} and Vogt, {Peter K.}",

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AU - Chang, Hwai Wen

AU - Aoki, Masahiro

AU - Fruman, David

AU - Auger, Kurt R.

AU - Bellacosa, Alfonso

AU - Tsichlis, Philip N.

AU - Cantley, Lewis C.

AU - Roberts, Thomas M.

AU - Vogt, Peter K.

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AB - The avian sarcoma virus 16 (ASV 16) is a retrovirus that induces hemangiosarcomas in chickens. Analysis of the ASV 16 genome revealed that it encodes an oncogene that is derived from the cellular gene for the catalytic subunit of phosphoinositide 3-kinase (PI 3-kinase). The gene is referred to as v-p3k, and like its cellular counterpart c-p3k, it is a potent transforming gene in cultured chicken embryo fibroblasts (CEFs). The products of the viral and cellular p3k genes have PI 3-kinase activity. CEFs transformed with either gene showed elevated levels of phosphatidylinositol 3,4-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate and activation of Akt kinase.

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Transformation of chicken cells by the gene encoding the catalytic subunit of PI 3-kinase

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