Transduction of Terminally Differentiated Neurons by Avian Sarcoma Virus

James G. Greger, Richard A. Katz, Konstantin Taganov, Glenn F. Rall, Anna Marie Skalka

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Recent studies have demonstrated that avian sarcoma virus (ASV) can transduce cycle-arrested cells. Here, we have assessed quantitatively the transduction efficiency of an ASV vector in naturally arrested mouse hippocampal neurons. This efficiency was determined by comparing the number of transduced cells after infection of differentiated neurons versus dividing progenitor cells. The results indicate that ASV is able to transduce these differentiated neurons efficiently and that this activity is not the result of infection of residual dividing cells. The transduction efficiency of the ASV vector was found to be intermediate between the relatively high and low efficiencies obtained with human immunodeficiency virus type I and murine leukemia virus vectors, respectively.

Original languageEnglish
Pages (from-to)4902-4906
Number of pages5
JournalJournal of Virology
Volume78
Issue number9
DOIs
StatePublished - May 2004

Keywords

  • Animals
  • Avian Sarcoma Viruses/genetics
  • Cell Differentiation
  • Cells, Cultured
  • Genetic Vectors
  • Green Fluorescent Proteins
  • Hippocampus/cytology
  • Luminescent Proteins/genetics
  • Mice
  • Neurons/cytology
  • Transduction, Genetic

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