TY - JOUR
T1 - Toll-like receptors 1 and 2 cooperatively mediate immune responses to curli, a common amyloid from enterobacterial biofilms
AU - Tükel, Çagla
AU - Nishimori, Jessalyn H.
AU - Wilson, R. Paul
AU - Winter, Maria G.
AU - Keestra, A. Marijke
AU - Van Putten, Jos P.M.
AU - Bäumler, Andreas J.
N1 - © 2010 Blackwell Publishing Ltd.
PY - 2010/9
Y1 - 2010/9
N2 - Responses to host amyloids and curli amyloid fibrils of Escherichia coli and Salmonella enterica serotype Typhimurium are mediated through Toll-like receptor (TLR) 2. Here we show that TLR2 alone was not sufficient for mediating responses to curli. Instead, transfection experiments with human cervical cancer (HeLa) cells and antibody-mediated inhibition of TLR signalling in human macrophage-like (THP-1) cells suggested that TLR2 interacts with TLR1 to recognize curli amyloid fibrils. TLR1/TLR2 also serves as a receptor for tri-acylated lipoproteins, which are produced by E. coli and other Gram-negative bacteria. Despite the presence of multiple TLR1/TLR2 ligands on intact bacterial cells, an inability to produce curli amyloid fibrils markedly reduced the ability of E. coli to induce TLR2-dependent responses in vitro and in vivo. Collectively, our data suggest that curli amyloid fibrils from enterobacterial biofilms significantly contribute to TLR1/TLR2-mediated host responses against intact bacterial cells.
AB - Responses to host amyloids and curli amyloid fibrils of Escherichia coli and Salmonella enterica serotype Typhimurium are mediated through Toll-like receptor (TLR) 2. Here we show that TLR2 alone was not sufficient for mediating responses to curli. Instead, transfection experiments with human cervical cancer (HeLa) cells and antibody-mediated inhibition of TLR signalling in human macrophage-like (THP-1) cells suggested that TLR2 interacts with TLR1 to recognize curli amyloid fibrils. TLR1/TLR2 also serves as a receptor for tri-acylated lipoproteins, which are produced by E. coli and other Gram-negative bacteria. Despite the presence of multiple TLR1/TLR2 ligands on intact bacterial cells, an inability to produce curli amyloid fibrils markedly reduced the ability of E. coli to induce TLR2-dependent responses in vitro and in vivo. Collectively, our data suggest that curli amyloid fibrils from enterobacterial biofilms significantly contribute to TLR1/TLR2-mediated host responses against intact bacterial cells.
KW - Amyloid/immunology
KW - Bacterial Proteins/immunology
KW - Biofilms
KW - Cell Line
KW - Epithelial Cells/immunology
KW - Escherichia coli/immunology
KW - Humans
KW - Macrophages/immunology
KW - Salmonella typhimurium/immunology
KW - Toll-Like Receptor 1/immunology
KW - Toll-Like Receptor 2/immunology
UR - http://www.scopus.com/inward/record.url?scp=78249262918&partnerID=8YFLogxK
U2 - 10.1111/j.1462-5822.2010.01485.x
DO - 10.1111/j.1462-5822.2010.01485.x
M3 - Article
C2 - 20497180
SN - 1462-5814
VL - 12
SP - 1495
EP - 1505
JO - Cellular Microbiology
JF - Cellular Microbiology
IS - 10
ER -