The Vi capsular polysaccharide prevents complement receptor 3-mediated clearance of Salmonella enterica serotype Typhi

R. Paul Wilson; Sebastian E. Winter; Alanna M. Spees; Maria G. Winter; Jessalyn H. Nishimori; Jesus F. Sanchez; Sean Paul Nuccio; Robert W. Crawford; Çagla Tükel; Andreas J. Bäumler

doi:10.1128/IAI.00961-10

The Vi capsular polysaccharide prevents complement receptor 3-mediated clearance of Salmonella enterica serotype Typhi

R. Paul Wilson, Sebastian E. Winter, Alanna M. Spees, Maria G. Winter, Jessalyn H. Nishimori, Jesus F. Sanchez, Sean Paul Nuccio, Robert W. Crawford, Çagla Tükel, Andreas J. Bäumler

Research output: Contribution to journal › Article › peer-review

87 Scopus citations

Abstract

Capsular polysaccharides are important virulence factors of invasive bacterial pathogens. Here we studied the role of the virulence (Vi) capsular polysaccharide of Salmonella enterica serotype Typhi (S. Typhi) in preventing innate immune recognition by complement. Comparison of capsulated S. Typhi with a noncapsulated mutant (ΔtviBCDE vexABCDE mutant) revealed that the Vi capsule interfered with complement component 3 (C3) deposition. Decreased complement fixation resulted in reduced bacterial binding to complement receptor 3 (CR3) on the surface of murine macrophages in vitro and decreased CR3-dependent clearance of Vi capsulated S. Typhi from the livers and spleens of mice. Opsonization of bacteria with immune serum prior to intraperitoneal infection increased clearance of capsulated S. Typhi from the liver. Our data suggest that the Vi capsule prevents CR3-dependent clearance, which can be overcome in part by a specific antibody response.

Original language	English
Pages (from-to)	830-837
Number of pages	8
Journal	Infection and Immunity
Volume	79
Issue number	2
DOIs	https://doi.org/10.1128/IAI.00961-10
State	Published - Feb 2011
Externally published	Yes

Access to Document

10.1128/IAI.00961-10

Cite this

@article{71980e0496ae40ca9d09ade4de51fcce,

title = "The Vi capsular polysaccharide prevents complement receptor 3-mediated clearance of Salmonella enterica serotype Typhi",

abstract = "Capsular polysaccharides are important virulence factors of invasive bacterial pathogens. Here we studied the role of the virulence (Vi) capsular polysaccharide of Salmonella enterica serotype Typhi (S. Typhi) in preventing innate immune recognition by complement. Comparison of capsulated S. Typhi with a noncapsulated mutant (ΔtviBCDE vexABCDE mutant) revealed that the Vi capsule interfered with complement component 3 (C3) deposition. Decreased complement fixation resulted in reduced bacterial binding to complement receptor 3 (CR3) on the surface of murine macrophages in vitro and decreased CR3-dependent clearance of Vi capsulated S. Typhi from the livers and spleens of mice. Opsonization of bacteria with immune serum prior to intraperitoneal infection increased clearance of capsulated S. Typhi from the liver. Our data suggest that the Vi capsule prevents CR3-dependent clearance, which can be overcome in part by a specific antibody response.",

author = "Wilson, {R. Paul} and Winter, {Sebastian E.} and Spees, {Alanna M.} and Winter, {Maria G.} and Nishimori, {Jessalyn H.} and Sanchez, {Jesus F.} and Nuccio, {Sean Paul} and Crawford, {Robert W.} and {\c C}agla T{\"u}kel and B{\"a}umler, {Andreas J.}",

year = "2011",

month = feb,

doi = "10.1128/IAI.00961-10",

language = "English",

volume = "79",

pages = "830--837",

journal = "Infection and Immunity",

issn = "0019-9567",

publisher = "American Society for Microbiology",

number = "2",

}

TY - JOUR

T1 - The Vi capsular polysaccharide prevents complement receptor 3-mediated clearance of Salmonella enterica serotype Typhi

AU - Wilson, R. Paul

AU - Winter, Sebastian E.

AU - Spees, Alanna M.

AU - Winter, Maria G.

AU - Nishimori, Jessalyn H.

AU - Sanchez, Jesus F.

AU - Nuccio, Sean Paul

AU - Crawford, Robert W.

AU - Tükel, Çagla

AU - Bäumler, Andreas J.

PY - 2011/2

Y1 - 2011/2

N2 - Capsular polysaccharides are important virulence factors of invasive bacterial pathogens. Here we studied the role of the virulence (Vi) capsular polysaccharide of Salmonella enterica serotype Typhi (S. Typhi) in preventing innate immune recognition by complement. Comparison of capsulated S. Typhi with a noncapsulated mutant (ΔtviBCDE vexABCDE mutant) revealed that the Vi capsule interfered with complement component 3 (C3) deposition. Decreased complement fixation resulted in reduced bacterial binding to complement receptor 3 (CR3) on the surface of murine macrophages in vitro and decreased CR3-dependent clearance of Vi capsulated S. Typhi from the livers and spleens of mice. Opsonization of bacteria with immune serum prior to intraperitoneal infection increased clearance of capsulated S. Typhi from the liver. Our data suggest that the Vi capsule prevents CR3-dependent clearance, which can be overcome in part by a specific antibody response.

AB - Capsular polysaccharides are important virulence factors of invasive bacterial pathogens. Here we studied the role of the virulence (Vi) capsular polysaccharide of Salmonella enterica serotype Typhi (S. Typhi) in preventing innate immune recognition by complement. Comparison of capsulated S. Typhi with a noncapsulated mutant (ΔtviBCDE vexABCDE mutant) revealed that the Vi capsule interfered with complement component 3 (C3) deposition. Decreased complement fixation resulted in reduced bacterial binding to complement receptor 3 (CR3) on the surface of murine macrophages in vitro and decreased CR3-dependent clearance of Vi capsulated S. Typhi from the livers and spleens of mice. Opsonization of bacteria with immune serum prior to intraperitoneal infection increased clearance of capsulated S. Typhi from the liver. Our data suggest that the Vi capsule prevents CR3-dependent clearance, which can be overcome in part by a specific antibody response.

UR - http://www.scopus.com/inward/record.url?scp=79251517374&partnerID=8YFLogxK

UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=purepublist2023&SrcAuth=WosAPI&KeyUT=WOS:000286462000029&DestLinkType=FullRecord&DestApp=WOS

U2 - 10.1128/IAI.00961-10

DO - 10.1128/IAI.00961-10

M3 - Article

C2 - 21098104

SN - 0019-9567

VL - 79

SP - 830

EP - 837

JO - Infection and Immunity

JF - Infection and Immunity

IS - 2

ER -

The Vi capsular polysaccharide prevents complement receptor 3-mediated clearance of Salmonella enterica serotype Typhi

Abstract

Access to Document

Other files and links

Fingerprint

Cite this