TY - JOUR
T1 - The tumor suppressor Mst1 promotes changes in the cellular redox state by phosphorylation and inactivation of peroxiredoxin-1 protein
AU - Rawat, Sonali Jalan
AU - Creasy, Caretha L.
AU - Peterson, Jeffrey R.
AU - Chernoff, Jonathan
PY - 2013/3/22
Y1 - 2013/3/22
N2 - The serine/threonine protein kinases Mst1 and Mst2 can be activated by cellular stressors including hydrogen peroxide. Using two independent protein interaction screens, we show that these kinases associate, in an oxidation-dependent manner, with Prdx1, an enzyme that regulates the cellular redox state by reducing hydrogen peroxide to water and oxygen. Mst1 inactivates Prdx1 by phosphorylating it at Thr-90 and Thr-183, leading to accumulation of hydrogen peroxide in cells. These results suggest that hydrogen peroxide-stimulated Mst1 activates a positive feedback loop to sustain an oxidizing cellular state.
AB - The serine/threonine protein kinases Mst1 and Mst2 can be activated by cellular stressors including hydrogen peroxide. Using two independent protein interaction screens, we show that these kinases associate, in an oxidation-dependent manner, with Prdx1, an enzyme that regulates the cellular redox state by reducing hydrogen peroxide to water and oxygen. Mst1 inactivates Prdx1 by phosphorylating it at Thr-90 and Thr-183, leading to accumulation of hydrogen peroxide in cells. These results suggest that hydrogen peroxide-stimulated Mst1 activates a positive feedback loop to sustain an oxidizing cellular state.
UR - http://www.scopus.com/inward/record.url?scp=84875451079&partnerID=8YFLogxK
U2 - 10.1074/jbc.M112.414524
DO - 10.1074/jbc.M112.414524
M3 - Article
C2 - 23386615
SN - 0021-9258
VL - 288
SP - 8762
EP - 8771
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 12
ER -