The structure of nucleosomal core particles within transcribed and repressed gene regions

Vasilyi M. Studitsky; Alexander V. Belyavsky; Anna F. Melnikova; Andrei D. Mirzabekov

doi:10.1093/nar/16.23.11187

The structure of nucleosomal core particles within transcribed and repressed gene regions

Vasilyi M. Studitsky, Alexander V. Belyavsky, Anna F. Melnikova, Andrei D. Mirzabekov

RAS - Engelhardt Institute of Molecular Biology

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

The arrangement of histones along DNA in nucleosomal core particles within transcribed heat shock gene (hsp 70) region and repressed insertion within ribosomal genes of Drosophila was analysed by using protein-DNA crosslinking methods combined with hybridization tests In addition, two-dimensional gel electrophoresis was employed to compare the overall nucleosomal shape and the nucleosomal DNA size. The arrangement of histones along DNA and general compactness of nucleosomes were shown to be rather similar in transcriptionally active and inactive genomic regions. On the other hand, nucleosomes with in transcriptionally active chromatin are characterized by a larger size of nucleosomal DNA produced by micrococcal nuclease digestion and some peculiarity in electrophoretic mobility.

Original language	English
Pages (from-to)	11187-11205
Number of pages	19
Journal	Nucleic Acids Research
Volume	16
Issue number	23
DOIs	https://doi.org/10.1093/nar/16.23.11187
State	Published - Dec 9 1988

Keywords

Animals
Chromatin/isolation & purification
Deoxyribonuclease I
Drosophila melanogaster
Heat-Shock Proteins/genetics
Histones/isolation & purification
Micrococcal Nuclease
Nucleic Acid Hybridization
Nucleosomes/analysis
Repressor Proteins/genetics
Transcription Factors/genetics
Transcription, Genetic

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title = "The structure of nucleosomal core particles within transcribed and repressed gene regions",

abstract = "The arrangement of histones along DNA in nucleosomal core particles within transcribed heat shock gene (hsp 70) region and repressed insertion within ribosomal genes of Drosophila was analysed by using protein-DNA crosslinking methods combined with hybridization tests In addition, two-dimensional gel electrophoresis was employed to compare the overall nucleosomal shape and the nucleosomal DNA size. The arrangement of histones along DNA and general compactness of nucleosomes were shown to be rather similar in transcriptionally active and inactive genomic regions. On the other hand, nucleosomes with in transcriptionally active chromatin are characterized by a larger size of nucleosomal DNA produced by micrococcal nuclease digestion and some peculiarity in electrophoretic mobility.",

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AU - Studitsky, Vasilyi M.

AU - Belyavsky, Alexander V.

AU - Melnikova, Anna F.

AU - Mirzabekov, Andrei D.

PY - 1988/12/9

Y1 - 1988/12/9

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KW - Animals

KW - Chromatin/isolation & purification

KW - Deoxyribonuclease I

KW - Drosophila melanogaster

KW - Heat-Shock Proteins/genetics

KW - Histones/isolation & purification

KW - Micrococcal Nuclease

KW - Nucleic Acid Hybridization

KW - Nucleosomes/analysis

KW - Repressor Proteins/genetics

KW - Transcription Factors/genetics

KW - Transcription, Genetic

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ER -

The structure of nucleosomal core particles within transcribed and repressed gene regions

Abstract

Keywords

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