Abstract
SH-PTP2, the vertebrate homolog of Drosophila corkscrew, associates with several activated growth factor receptors, but its biological function is unknown. We assayed the effects of injection of wild-type and mutant SH-PTP2 RNAs on Xenopus embryogenesis. An internal phosphatase domain deletion (ΔP) acts as a dominant negative mutant, causing severe posterior truncations. This phenotype is rescued by SH-PTP2, but not by the closely related SH-PTP1. In ectodermal explants, ΔP blocks fibroblast growth factor (FGF)- and activin-mediated induction of mesoderm and FGF-induced mitogen-activated protein (MAP) kinase activation. Our results indicate that SH-PTP2 is required for early vertebrate development, acting as a positive component in FGF signaling downstream of the FGF receptor and upstream of MAP kinase.
| Original language | English |
|---|---|
| Pages (from-to) | 473-483 |
| Number of pages | 11 |
| Journal | Cell |
| Volume | 80 |
| Issue number | 3 |
| DOIs | |
| State | Published - Feb 10 1995 |
Keywords
- Activins
- Amino Acid Sequence
- Animals
- Base Sequence
- Calcium-Calmodulin-Dependent Protein Kinases/genetics
- Cloning, Molecular
- Culture Techniques
- Ectoderm/drug effects
- Embryonic Induction/drug effects
- Female
- Fibroblast Growth Factor 2/pharmacology
- Inhibins/pharmacology
- Intracellular Signaling Peptides and Proteins
- Mesoderm/drug effects
- Molecular Sequence Data
- Protein Tyrosine Phosphatase, Non-Receptor Type 11
- Protein Tyrosine Phosphatase, Non-Receptor Type 6
- Protein Tyrosine Phosphatases/genetics
- RNA, Messenger/genetics
- Sequence Analysis, DNA
- Sequence Deletion/physiology
- Xenopus