The role of c-myc protooncogene in chronic myelogenous leukemia

M. Nieborowska-Skorska, M. Z. Ratajczak, B. Calabretta, T. Skorski

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13 Scopus citations

Abstract

c-Myc transcriptional factor encoded by c-myc protooncogene plays an important role in the regulation of cell cycle. It was also established that c-Myc is important for the transformation of fibroblasts and murine bone marrow cells induced by BCR/ABL tyrosine kinase encoded by bcr/abl oncogene localized on Philadelphia-chromosome (Ph1). The role of c-Myc in the proliferation of the leukemic cells was not known. Therefore, we examined the effect of c-Myc protein downregulation, using antisense oligodeoxynucleotides, on the growth of the BCR/ABL-dependent cell line and chronic myelogenous leukemia (CML) patients cells. Downregulation of c-Myc expression caused complete inhibition of the proliferation of BCR/ABL-dependent BV173 cell line and 50-70% inhibition of the colony formation of CML-cells. These results suggests that c-Myc cooperates with BCR/ABL and is necessary for the growth of Ph1-positive leukemias.

Original languageEnglish
Pages (from-to)231-234
Number of pages4
JournalFolia Histochemica et Cytobiologica
Volume32
Issue number4
StatePublished - 1994

Keywords

  • antisense nucleotides
  • leukemic cells
  • oncogenes

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