The interferon-inducible antiviral protein Daxx is not essential for interferon-mediated protection against avian sarcoma virus

Kelsey A. Haugh, Natalia Shalginskikh, Shoko Nogusa, Anna Marie Skalka, Richard A. Katz, Siddharth Balachandran

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Background: The antiviral protein Daxx acts as a restriction factor of avian sarcoma virus (ASV; Retroviridae) in mammalian cells by promoting epigenetic silencing of integrated proviral DNA. Although Daxx is encoded by a type I (α/β) interferon-stimulated gene, the requirement for Daxx in the interferon anti-retroviral response has not been elucidated. In this report, we describe the results of experiments designed to investigate the role of Daxx in the type I interferon-induced anti-ASV response. Findings. Using an ASV reporter system, we show that type I interferons are potent inhibitors of ASV replication. We demonstrate that, while Daxx is necessary to silence ASV gene expression in the absence of interferons, type I interferons are fully-capable of inducing an antiviral state in the absence of Daxx. Conclusions: These results provide evidence that Daxx is not essential for the anti-ASV interferon response in mammalian cells, and that interferons deploy multiple, redundant antiviral mechanisms to protect cells from ASV.

Original languageEnglish
Article number100
Pages (from-to)100
JournalVirology Journal
Volume11
Issue number1
DOIs
StatePublished - May 28 2014

Keywords

  • Adaptor Proteins, Signal Transducing/immunology
  • Animals
  • Avian Sarcoma Viruses/immunology
  • Birds
  • Cell Line
  • Co-Repressor Proteins
  • Humans
  • Interferon Type I/immunology
  • Molecular Chaperones
  • Nuclear Proteins/immunology
  • Virus Replication

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