TY - JOUR
T1 - The interferon-inducible antiviral protein Daxx is not essential for interferon-mediated protection against avian sarcoma virus
AU - Haugh, Kelsey A.
AU - Shalginskikh, Natalia
AU - Nogusa, Shoko
AU - Skalka, Anna Marie
AU - Katz, Richard A.
AU - Balachandran, Siddharth
PY - 2014/5/28
Y1 - 2014/5/28
N2 - Background: The antiviral protein Daxx acts as a restriction factor of avian sarcoma virus (ASV; Retroviridae) in mammalian cells by promoting epigenetic silencing of integrated proviral DNA. Although Daxx is encoded by a type I (α/β) interferon-stimulated gene, the requirement for Daxx in the interferon anti-retroviral response has not been elucidated. In this report, we describe the results of experiments designed to investigate the role of Daxx in the type I interferon-induced anti-ASV response. Findings. Using an ASV reporter system, we show that type I interferons are potent inhibitors of ASV replication. We demonstrate that, while Daxx is necessary to silence ASV gene expression in the absence of interferons, type I interferons are fully-capable of inducing an antiviral state in the absence of Daxx. Conclusions: These results provide evidence that Daxx is not essential for the anti-ASV interferon response in mammalian cells, and that interferons deploy multiple, redundant antiviral mechanisms to protect cells from ASV.
AB - Background: The antiviral protein Daxx acts as a restriction factor of avian sarcoma virus (ASV; Retroviridae) in mammalian cells by promoting epigenetic silencing of integrated proviral DNA. Although Daxx is encoded by a type I (α/β) interferon-stimulated gene, the requirement for Daxx in the interferon anti-retroviral response has not been elucidated. In this report, we describe the results of experiments designed to investigate the role of Daxx in the type I interferon-induced anti-ASV response. Findings. Using an ASV reporter system, we show that type I interferons are potent inhibitors of ASV replication. We demonstrate that, while Daxx is necessary to silence ASV gene expression in the absence of interferons, type I interferons are fully-capable of inducing an antiviral state in the absence of Daxx. Conclusions: These results provide evidence that Daxx is not essential for the anti-ASV interferon response in mammalian cells, and that interferons deploy multiple, redundant antiviral mechanisms to protect cells from ASV.
KW - Adaptor Proteins, Signal Transducing/immunology
KW - Animals
KW - Avian Sarcoma Viruses/immunology
KW - Birds
KW - Cell Line
KW - Co-Repressor Proteins
KW - Humans
KW - Interferon Type I/immunology
KW - Molecular Chaperones
KW - Nuclear Proteins/immunology
KW - Virus Replication
UR - http://www.scopus.com/inward/record.url?scp=84902240171&partnerID=8YFLogxK
UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=purepublist2023&SrcAuth=WosAPI&KeyUT=WOS:000337308300001&DestLinkType=FullRecord&DestApp=WOS
U2 - 10.1186/1743-422X-11-100
DO - 10.1186/1743-422X-11-100
M3 - Article
C2 - 24884573
SN - 1743-422X
VL - 11
SP - 100
JO - Virology Journal
JF - Virology Journal
IS - 1
M1 - 100
ER -