TY - JOUR
T1 - The influence of phosphorothioate oligodeoxynucleotides on various organs in vivo
AU - Nieborowska-Skórska, Małgorzata
AU - Białek, Andrzej P.
AU - Nicolaides, Nicolas C.
AU - Lozzo, Renato V.
AU - Kawalec, Maciej
AU - Calabretta, Bruno
AU - Kawiak, Jerzy
AU - Marlicz, Krzysztof
AU - Skórski, Tomasz
PY - 1996
Y1 - 1996
N2 - To characterize the distribution and toxicity of phosphorothioate antisense oligodeoxynucleotides ([S]ODNs) in vivo, the mice, previously injected with BV173 leukemic cells (Philadelphia chromosome-positive chronic myeloid leukemia blast-crisis), received intravenously 26-mer BCR-ABL antisense oligodeoxynucleotides (1 mg/mouse/day) for 9 consecutive days. Our investigation revealed that [S]ODNs were distributed to almost all organs except the brain with the highest level in the liver, spleen and kidneys. They were also detected in CD10+ leukemic cells isolated from spleen and bone marrow. Intracellular distribution assay showed the presence of [S]ODNs most prominently in nuclear and cytoplasmic fractions. Our data demonstrated no significant toxicity of [S]ODNs except the increase in spleen weight.
AB - To characterize the distribution and toxicity of phosphorothioate antisense oligodeoxynucleotides ([S]ODNs) in vivo, the mice, previously injected with BV173 leukemic cells (Philadelphia chromosome-positive chronic myeloid leukemia blast-crisis), received intravenously 26-mer BCR-ABL antisense oligodeoxynucleotides (1 mg/mouse/day) for 9 consecutive days. Our investigation revealed that [S]ODNs were distributed to almost all organs except the brain with the highest level in the liver, spleen and kidneys. They were also detected in CD10+ leukemic cells isolated from spleen and bone marrow. Intracellular distribution assay showed the presence of [S]ODNs most prominently in nuclear and cytoplasmic fractions. Our data demonstrated no significant toxicity of [S]ODNs except the increase in spleen weight.
KW - Antisense
KW - Mice
KW - Oligodeoxynucleotides
KW - Toxicity
UR - https://www.scopus.com/pages/publications/0029818580
UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=purepublist2023&SrcAuth=WosAPI&KeyUT=WOS:A1996UY41100002&DestLinkType=FullRecord&DestApp=WOS
M3 - Article
C2 - 8875213
SN - 0015-5586
VL - 34
SP - 69
EP - 73
JO - Folia Histochemica et Cytobiologica
JF - Folia Histochemica et Cytobiologica
IS - 2
ER -