The FHIT gene, spanning the chromosome 3p14.2 fragile site and renal carcinoma-associated t(3;8) breakpoint, is abnormal in digestive tract cancers

Masataka Ohta; Hiroshi Inoue; Maria Grazia Cotticelli; Kumar Kastury; Raffaele Baffa; Juan Palazzo; Zurab Siprashvili; Masaki Mori; Peter McCue; Teresa Druck; Carlo M. Croce; Kay Huebner

doi:10.1016/S0092-8674(00)81034-X

The FHIT gene, spanning the chromosome 3p14.2 fragile site and renal carcinoma-associated t(3;8) breakpoint, is abnormal in digestive tract cancers

Masataka Ohta, Hiroshi Inoue, Maria Grazia Cotticelli, Kumar Kastury, Raffaele Baffa, Juan Palazzo, Zurab Siprashvili, Masaki Mori, Peter McCue, Teresa Druck, Carlo M. Croce, Kay Huebner

Pathology

Research output: Contribution to journal › Article › peer-review

1054 Scopus citations

Abstract

A 200-300 kb region of chromosome 3p14.2, including the fragile site locus FRA3B, is homozygously deleted in multiple tumor-derived cell lines. Exon amplification from cosmids covering this deleted region allowed identification of the human FHIT gene, a member of the histidine triad gene family, which encodes a protein with 69% similarity to an S. pombe enzyme, diadenosine 5', 5'' P¹, P⁴-tetraphosphate asymmetrical hydrolase. The FHIT locus is composed of ten exons distributed over at least 500 kb, with three 5' untranslated exons centromeric to the renal carcinoma associated 3p14.2 breakpoint, the remaining exons telomeric to this translocation breakpoint, and exon 5 within the homozygously deleted fragile region. Aberrant transcripts of the FHIT locus were found in ~50% of esophageal, stomach, and colon carcinomas.

Original language	English
Pages (from-to)	587-597
Number of pages	11
Journal	Cell
Volume	84
Issue number	4
DOIs	https://doi.org/10.1016/S0092-8674(00)81034-X
State	Published - Feb 23 1996

Keywords

Acid Anhydride Hydrolases
Amino Acid Sequence
Base Sequence
Chromosome Fragile Sites
Chromosome Fragility
Chromosome Mapping
Chromosomes, Human, Pair 3
Chromosomes, Human, Pair 8
Colonic Neoplasms/genetics
Cosmids/genetics
DNA, Complementary/genetics
Esophageal Neoplasms/genetics
Exons/genetics
Gastrointestinal Neoplasms/genetics
Gene Deletion
Genes, Neoplasm
Humans
Hydrolases
Kidney Neoplasms/genetics
Molecular Sequence Data
Neoplasm Proteins
Polymerase Chain Reaction
Proteins/genetics
RNA, Messenger/genetics
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Stomach Neoplasms/genetics
Translocation, Genetic
Tumor Cells, Cultured/physiology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0092-8674(00)81034-X

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Ohta, M., Inoue, H., Cotticelli, M. G., Kastury, K., Baffa, R., Palazzo, J., Siprashvili, Z., Mori, M., McCue, P., Druck, T., Croce, C. M., & Huebner, K. (1996). The FHIT gene, spanning the chromosome 3p14.2 fragile site and renal carcinoma-associated t(3;8) breakpoint, is abnormal in digestive tract cancers. Cell, 84(4), 587-597. https://doi.org/10.1016/S0092-8674(00)81034-X

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title = "The FHIT gene, spanning the chromosome 3p14.2 fragile site and renal carcinoma-associated t(3;8) breakpoint, is abnormal in digestive tract cancers",

abstract = "A 200-300 kb region of chromosome 3p14.2, including the fragile site locus FRA3B, is homozygously deleted in multiple tumor-derived cell lines. Exon amplification from cosmids covering this deleted region allowed identification of the human FHIT gene, a member of the histidine triad gene family, which encodes a protein with 69% similarity to an S. pombe enzyme, diadenosine 5', 5'' P1, P4-tetraphosphate asymmetrical hydrolase. The FHIT locus is composed of ten exons distributed over at least 500 kb, with three 5' untranslated exons centromeric to the renal carcinoma associated 3p14.2 breakpoint, the remaining exons telomeric to this translocation breakpoint, and exon 5 within the homozygously deleted fragile region. Aberrant transcripts of the FHIT locus were found in ~50% of esophageal, stomach, and colon carcinomas.",

keywords = "Acid Anhydride Hydrolases, Amino Acid Sequence, Base Sequence, Chromosome Fragile Sites, Chromosome Fragility, Chromosome Mapping, Chromosomes, Human, Pair 3, Chromosomes, Human, Pair 8, Colonic Neoplasms/genetics, Cosmids/genetics, DNA, Complementary/genetics, Esophageal Neoplasms/genetics, Exons/genetics, Gastrointestinal Neoplasms/genetics, Gene Deletion, Genes, Neoplasm, Humans, Hydrolases, Kidney Neoplasms/genetics, Molecular Sequence Data, Neoplasm Proteins, Polymerase Chain Reaction, Proteins/genetics, RNA, Messenger/genetics, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Stomach Neoplasms/genetics, Translocation, Genetic, Tumor Cells, Cultured/physiology",

author = "Masataka Ohta and Hiroshi Inoue and Cotticelli, {Maria Grazia} and Kumar Kastury and Raffaele Baffa and Juan Palazzo and Zurab Siprashvili and Masaki Mori and Peter McCue and Teresa Druck and Croce, {Carlo M.} and Kay Huebner",

year = "1996",

month = feb,

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doi = "10.1016/S0092-8674(00)81034-X",

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Ohta, M, Inoue, H, Cotticelli, MG, Kastury, K, Baffa, R, Palazzo, J, Siprashvili, Z, Mori, M, McCue, P, Druck, T, Croce, CM & Huebner, K 1996, 'The FHIT gene, spanning the chromosome 3p14.2 fragile site and renal carcinoma-associated t(3;8) breakpoint, is abnormal in digestive tract cancers', Cell, vol. 84, no. 4, pp. 587-597. https://doi.org/10.1016/S0092-8674(00)81034-X

TY - JOUR

T1 - The FHIT gene, spanning the chromosome 3p14.2 fragile site and renal carcinoma-associated t(3;8) breakpoint, is abnormal in digestive tract cancers

AU - Ohta, Masataka

AU - Inoue, Hiroshi

AU - Cotticelli, Maria Grazia

AU - Kastury, Kumar

AU - Baffa, Raffaele

AU - Palazzo, Juan

AU - Siprashvili, Zurab

AU - Mori, Masaki

AU - McCue, Peter

AU - Druck, Teresa

AU - Croce, Carlo M.

AU - Huebner, Kay

PY - 1996/2/23

Y1 - 1996/2/23

N2 - A 200-300 kb region of chromosome 3p14.2, including the fragile site locus FRA3B, is homozygously deleted in multiple tumor-derived cell lines. Exon amplification from cosmids covering this deleted region allowed identification of the human FHIT gene, a member of the histidine triad gene family, which encodes a protein with 69% similarity to an S. pombe enzyme, diadenosine 5', 5'' P1, P4-tetraphosphate asymmetrical hydrolase. The FHIT locus is composed of ten exons distributed over at least 500 kb, with three 5' untranslated exons centromeric to the renal carcinoma associated 3p14.2 breakpoint, the remaining exons telomeric to this translocation breakpoint, and exon 5 within the homozygously deleted fragile region. Aberrant transcripts of the FHIT locus were found in ~50% of esophageal, stomach, and colon carcinomas.

AB - A 200-300 kb region of chromosome 3p14.2, including the fragile site locus FRA3B, is homozygously deleted in multiple tumor-derived cell lines. Exon amplification from cosmids covering this deleted region allowed identification of the human FHIT gene, a member of the histidine triad gene family, which encodes a protein with 69% similarity to an S. pombe enzyme, diadenosine 5', 5'' P1, P4-tetraphosphate asymmetrical hydrolase. The FHIT locus is composed of ten exons distributed over at least 500 kb, with three 5' untranslated exons centromeric to the renal carcinoma associated 3p14.2 breakpoint, the remaining exons telomeric to this translocation breakpoint, and exon 5 within the homozygously deleted fragile region. Aberrant transcripts of the FHIT locus were found in ~50% of esophageal, stomach, and colon carcinomas.

KW - Acid Anhydride Hydrolases

KW - Amino Acid Sequence

KW - Base Sequence

KW - Chromosome Fragile Sites

KW - Chromosome Fragility

KW - Chromosome Mapping

KW - Chromosomes, Human, Pair 3

KW - Chromosomes, Human, Pair 8

KW - Colonic Neoplasms/genetics

KW - Cosmids/genetics

KW - DNA, Complementary/genetics

KW - Esophageal Neoplasms/genetics

KW - Exons/genetics

KW - Gastrointestinal Neoplasms/genetics

KW - Gene Deletion

KW - Genes, Neoplasm

KW - Humans

KW - Hydrolases

KW - Kidney Neoplasms/genetics

KW - Molecular Sequence Data

KW - Neoplasm Proteins

KW - Polymerase Chain Reaction

KW - Proteins/genetics

KW - RNA, Messenger/genetics

KW - Sequence Analysis, DNA

KW - Sequence Homology, Amino Acid

KW - Stomach Neoplasms/genetics

KW - Translocation, Genetic

KW - Tumor Cells, Cultured/physiology

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U2 - 10.1016/S0092-8674(00)81034-X

DO - 10.1016/S0092-8674(00)81034-X

M3 - Article

C2 - 8598045

AN - SCOPUS:13344279424

SN - 0092-8674

VL - 84

SP - 587

EP - 597

JO - Cell

JF - Cell

IS - 4

ER -

The FHIT gene, spanning the chromosome 3p14.2 fragile site and renal carcinoma-associated t(3;8) breakpoint, is abnormal in digestive tract cancers

Abstract

Keywords

UN SDGs

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