The continued evolution of two-hybrid screening approaches in yeast: How to outwit different preys with different baits

Sarah J. Fashena, Ilya Serebriiskii, Erica A. Golemis

Research output: Contribution to journalReview articlepeer-review

56 Scopus citations

Abstract

The original two-hybrid system, an experimental approach designed to detect protein interactions, exploited the modular nature of many transcription factors. It has provided the intellectual and technical seed for the evolution of an array of innovative approaches, the application of which broadens the scope of experimentally feasible questions to include the interaction of proteins with diverse binding partners. The available array of modified and alternative approaches facilitates the analysis of complex cellular machinery and signaling networks that rely on multiple protein interactions. Such advances have facilitated the functional analysis of proteins on the genome level, a feat considered untenable a decade ago. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)1-14
Number of pages14
JournalGene
Volume250
Issue number1-2
DOIs
StatePublished - May 30 2000

Keywords

  • Gal4p and LexA
  • Protein interaction
  • S. cerevisiae

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