The cloning and characterization of human MyD88: A member of an IL-1 receptor related family

Timothy P. Bonnert; Kirsten E. Garka; Patricia Parnet; Gonosuke Sonoda; Joseph R. Testa; John E. Sims

doi:10.1016/S0014-5793(96)01506-2

The cloning and characterization of human MyD88: A member of an IL-1 receptor related family

Timothy P. Bonnert, Kirsten E. Garka, Patricia Parnet, Gonosuke Sonoda, Joseph R. Testa, John E. Sims

Cancer Prevention and Control (CPC)

Research output: Contribution to journal › Article › peer-review

121 Scopus citations

Abstract

Murine MyD88, an RNA with homology both to the interleukin-1 receptor signaling domain and to 'death-domains', is rapidly upregulated during differentiation of the myeloleukemic cell line M1. We have cloned the human homologue of murine MyD88 and re-evaluated the murine sequence. The open reading frame for both species encodes a 296 amino acid protein, which for murine MyD88 is 53 amino acids longer than originally published. Human MyD88 cDNA is encoded by 5 exons, and maps to chromosome 3p21.3-p22 by fluorescence in situ hybridization (FISH). Overexpression of the death domain region leads to transcriptional activation of the IL-8 promoter.

Original language	English
Pages (from-to)	81-84
Number of pages	4
Journal	FEBS Letters
Volume	402
Issue number	1
DOIs	https://doi.org/10.1016/S0014-5793(96)01506-2
State	Published - Feb 3 1997

Keywords

Adaptor Proteins, Signal Transducing
Amino Acid Sequence
Animals
Antigens, Differentiation
Base Sequence
COS Cells
Chromosome Mapping
Chromosomes, Human, Pair 3
DNA, Complementary/genetics
Exons
Gene Expression
Humans
In Situ Hybridization, Fluorescence
Interleukin-8/genetics
Introns
Mice
Molecular Sequence Data
Myeloid Differentiation Factor 88
Oligonucleotides, Antisense
Promoter Regions, Genetic
Proteins/chemistry
RNA, Messenger/genetics
Receptors, Immunologic
Receptors, Interleukin-1/chemistry
Transfection

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10.1016/S0014-5793(96)01506-2

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title = "The cloning and characterization of human MyD88: A member of an IL-1 receptor related family",

abstract = "Murine MyD88, an RNA with homology both to the interleukin-1 receptor signaling domain and to 'death-domains', is rapidly upregulated during differentiation of the myeloleukemic cell line M1. We have cloned the human homologue of murine MyD88 and re-evaluated the murine sequence. The open reading frame for both species encodes a 296 amino acid protein, which for murine MyD88 is 53 amino acids longer than originally published. Human MyD88 cDNA is encoded by 5 exons, and maps to chromosome 3p21.3-p22 by fluorescence in situ hybridization (FISH). Overexpression of the death domain region leads to transcriptional activation of the IL-8 promoter.",

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author = "Bonnert, {Timothy P.} and Garka, {Kirsten E.} and Patricia Parnet and Gonosuke Sonoda and Testa, {Joseph R.} and Sims, {John E.}",

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doi = "10.1016/S0014-5793(96)01506-2",

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AU - Garka, Kirsten E.

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AU - Sonoda, Gonosuke

AU - Testa, Joseph R.

AU - Sims, John E.

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N2 - Murine MyD88, an RNA with homology both to the interleukin-1 receptor signaling domain and to 'death-domains', is rapidly upregulated during differentiation of the myeloleukemic cell line M1. We have cloned the human homologue of murine MyD88 and re-evaluated the murine sequence. The open reading frame for both species encodes a 296 amino acid protein, which for murine MyD88 is 53 amino acids longer than originally published. Human MyD88 cDNA is encoded by 5 exons, and maps to chromosome 3p21.3-p22 by fluorescence in situ hybridization (FISH). Overexpression of the death domain region leads to transcriptional activation of the IL-8 promoter.

AB - Murine MyD88, an RNA with homology both to the interleukin-1 receptor signaling domain and to 'death-domains', is rapidly upregulated during differentiation of the myeloleukemic cell line M1. We have cloned the human homologue of murine MyD88 and re-evaluated the murine sequence. The open reading frame for both species encodes a 296 amino acid protein, which for murine MyD88 is 53 amino acids longer than originally published. Human MyD88 cDNA is encoded by 5 exons, and maps to chromosome 3p21.3-p22 by fluorescence in situ hybridization (FISH). Overexpression of the death domain region leads to transcriptional activation of the IL-8 promoter.

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KW - Antigens, Differentiation

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KW - DNA, Complementary/genetics

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KW - In Situ Hybridization, Fluorescence

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KW - Mice

KW - Molecular Sequence Data

KW - Myeloid Differentiation Factor 88

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KW - Promoter Regions, Genetic

KW - Proteins/chemistry

KW - RNA, Messenger/genetics

KW - Receptors, Immunologic

KW - Receptors, Interleukin-1/chemistry

KW - Transfection

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The cloning and characterization of human MyD88: A member of an IL-1 receptor related family

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