Abstract
We report here experimental evidence indicating that the p21(CIP), the universal inhibitor of cyclin-dependent protein kinases, general inhibitorCDKs is a substrate oof cyclin A-cdk2. The evidence comes from phosphorylation experiments in which the endogenous p21CIP present in using the original cyclin A-cdk2 complexes immunoprecipitated from HeLa cells extracts can be phosphorylated by the cdk2 of the same complexes. In vitro experiments showing that reconstituted GSTcyclin A-GSTcdk2 complexes from phosphorylate recombinant GSTp21(CIP) confirms that p21(CIP) is a cyclin A-cdk2 substrate.
| Original language | English |
|---|---|
| Pages (from-to) | 434-438 |
| Number of pages | 5 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 241 |
| Issue number | 2 |
| DOIs | |
| State | Published - Dec 18 1997 |
| Externally published | Yes |