TY - JOUR
T1 - The adaptor protein Nck links receptor tyrosine kinases with the serine- threonine kinase Pak1
AU - Galisteo, Maria L.
AU - Chernoff, Jonathan
AU - Su, Yi Chi
AU - Skolnik, Edward Y.
AU - Schlessinger, Joseph
N1 - Times Cited: 130 English Article VE477 J BIOL CHEM
PY - 1996
Y1 - 1996
N2 - Nck is an adaptor protein composed of a single SH2 domain and three SH3 domains. Upon growth factor stimulation, Nck is recruited to receptor tyrosine kinases via its SH2 domain, probably initiating one or more signaling cascades. In this report, we show that Nck is bound in living cells to the serine-threonine kinase Pak1. The association between Nck and Pak1 is mediated by the second SH3 domain of Nck and a proline-rich sequence in the amino terminus of Pak1. We also show that Pak1 is recruited by activated epidermal growth factor (EGF) and platelet-derived growth factor receptors. Moreover, Pak1 kinase activity is increased in response to EGF in HeLa cells transfected with human Pak1, and the kinase activity was enhanced when Nck was co-transfected. It is concluded that Nck links receptor tyrosine kinases with Pak1 and is probably involved in targeting and regulation of Pak1 activity.
AB - Nck is an adaptor protein composed of a single SH2 domain and three SH3 domains. Upon growth factor stimulation, Nck is recruited to receptor tyrosine kinases via its SH2 domain, probably initiating one or more signaling cascades. In this report, we show that Nck is bound in living cells to the serine-threonine kinase Pak1. The association between Nck and Pak1 is mediated by the second SH3 domain of Nck and a proline-rich sequence in the amino terminus of Pak1. We also show that Pak1 is recruited by activated epidermal growth factor (EGF) and platelet-derived growth factor receptors. Moreover, Pak1 kinase activity is increased in response to EGF in HeLa cells transfected with human Pak1, and the kinase activity was enhanced when Nck was co-transfected. It is concluded that Nck links receptor tyrosine kinases with Pak1 and is probably involved in targeting and regulation of Pak1 activity.
KW - Adaptor Proteins, Signal Transducing
KW - Amino Acid Sequence
KW - Animals
KW - Cell Line
KW - ErbB Receptors/metabolism
KW - HeLa Cells
KW - Humans
KW - Molecular Sequence Data
KW - Oncogene Proteins/metabolism
KW - Protein Binding
KW - Protein Serine-Threonine Kinases/metabolism
KW - Rats
KW - Receptor Protein-Tyrosine Kinases/metabolism
KW - Receptors, Platelet-Derived Growth Factor/metabolism
KW - Signal Transduction
KW - p21-Activated Kinases
UR - http://www.scopus.com/inward/record.url?scp=0029830139&partnerID=8YFLogxK
UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=purepublist2023&SrcAuth=WosAPI&KeyUT=WOS:A1996VE47700007&DestLinkType=FullRecord&DestApp=WOS
U2 - 10.1074/jbc.271.35.20997
DO - 10.1074/jbc.271.35.20997
M3 - Article
C2 - 8798379
SN - 0021-9258
VL - 271
SP - 20997
EP - 21000
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 35
ER -