Study of RNA polymerase transcription through nucleosome using the cryo-electron microscopy approach

O. I. Volokh; F. K. Hsieh; M. G. Karlova; E. S. Trifonova; V. M. Studitsky; O. S. Sokolova

doi:10.3103/S0096392516010120

Study of RNA polymerase transcription through nucleosome using the cryo-electron microscopy approach

O. I. Volokh, F. K. Hsieh, M. G. Karlova, E. S. Trifonova, V. M. Studitsky, O. S. Sokolova

Research output: Contribution to journal › Article › peer-review

Abstract

A promising and reliable method for the analysis of macromolecule structures is capturing images using cryo-electron transmission microscopy and consequently performing three-dimensional reconstructions. In this study, using cryo-electron microscopy, we analyzed the structure of the complex, formed by RNA polymerase stalled at position +42 during its transcription through the nucleosome. We obtained both projection images and a three-dimensional structure of the EC-42 complex at 2.5 nm resolution. This allowed us to confirm the conformational integrity of the nucleosome during the passage of the RNA polymerase.

Original language	English
Pages (from-to)	34-38
Number of pages	5
Journal	Moscow University Biological Sciences Bulletin
Volume	71
Issue number	1
DOIs	https://doi.org/10.3103/S0096392516010120
State	Published - Jan 1 2016

Keywords

RNA polymerase
cryo-electron microscopy
nucleosome
transcription

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10.3103/S0096392516010120

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title = "Study of RNA polymerase transcription through nucleosome using the cryo-electron microscopy approach",

abstract = "A promising and reliable method for the analysis of macromolecule structures is capturing images using cryo-electron transmission microscopy and consequently performing three-dimensional reconstructions. In this study, using cryo-electron microscopy, we analyzed the structure of the complex, formed by RNA polymerase stalled at position +42 during its transcription through the nucleosome. We obtained both projection images and a three-dimensional structure of the EC-42 complex at 2.5 nm resolution. This allowed us to confirm the conformational integrity of the nucleosome during the passage of the RNA polymerase.",

keywords = "RNA polymerase, cryo-electron microscopy, nucleosome, transcription",

author = "Volokh, \{O. I.\} and Hsieh, \{F. K.\} and Karlova, \{M. G.\} and Trifonova, \{E. S.\} and Studitsky, \{V. M.\} and Sokolova, \{O. S.\}",

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doi = "10.3103/S0096392516010120",

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AU - Volokh, O. I.

AU - Hsieh, F. K.

AU - Karlova, M. G.

AU - Trifonova, E. S.

AU - Studitsky, V. M.

AU - Sokolova, O. S.

PY - 2016/1/1

Y1 - 2016/1/1

N2 - A promising and reliable method for the analysis of macromolecule structures is capturing images using cryo-electron transmission microscopy and consequently performing three-dimensional reconstructions. In this study, using cryo-electron microscopy, we analyzed the structure of the complex, formed by RNA polymerase stalled at position +42 during its transcription through the nucleosome. We obtained both projection images and a three-dimensional structure of the EC-42 complex at 2.5 nm resolution. This allowed us to confirm the conformational integrity of the nucleosome during the passage of the RNA polymerase.

AB - A promising and reliable method for the analysis of macromolecule structures is capturing images using cryo-electron transmission microscopy and consequently performing three-dimensional reconstructions. In this study, using cryo-electron microscopy, we analyzed the structure of the complex, formed by RNA polymerase stalled at position +42 during its transcription through the nucleosome. We obtained both projection images and a three-dimensional structure of the EC-42 complex at 2.5 nm resolution. This allowed us to confirm the conformational integrity of the nucleosome during the passage of the RNA polymerase.

KW - RNA polymerase

KW - cryo-electron microscopy

KW - nucleosome

KW - transcription

UR - https://www.scopus.com/pages/publications/84964699501

U2 - 10.3103/S0096392516010120

DO - 10.3103/S0096392516010120

M3 - Article

AN - SCOPUS:84964699501

SN - 0096-3925

VL - 71

SP - 34

EP - 38

JO - Moscow University Biological Sciences Bulletin

JF - Moscow University Biological Sciences Bulletin

IS - 1

ER -

Study of RNA polymerase transcription through nucleosome using the cryo-electron microscopy approach

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