Structural studies of chromatin remodeling factors

O. I. Volokh; N. I. Derkacheva; V. M. Studitsky; O. S. Sokolova

doi:10.1134/S0026893316060212

Structural studies of chromatin remodeling factors

O. I. Volokh, N. I. Derkacheva, V. M. Studitsky, O. S. Sokolova

Research output: Contribution to journal › Review article › peer-review

3 Scopus citations

Abstract

Changes of chromatin structure require participation of chromatin remodeling factors (CRFs), which are ATP-dependent multisubunit complexes that change the structure of the nucleosome without covalently modifying its components. CRFs act together with other protein factors to regulate the extent of chromatin condensation. Four CRF families are currently distinguished based on their structural and biochemical characteristics: SWI/SNF, ISWI, Mi-2/CHD, and SWR/INO80. X-ray diffraction analysis and electron microscopy are the main methods to obtain structural information about macromolecules. CRFs are difficult to obtain in crystal because of their large sizes and structural heterogeneity, and transmission electron microscopy (TEM) is mostly employed in their structural studies. The review considers all structures obtained for CRFs by TEM and discusses several models of CRF–nucleosome interactions.

Original language	English
Pages (from-to)	812-822
Number of pages	11
Journal	Molecular Biology
Volume	50
Issue number	6
DOIs	https://doi.org/10.1134/S0026893316060212
State	Published - Nov 2016

Keywords

Chromatin
Chromatin remodeling factors
Nucleosome
Transcription
Transmission electron microscopy

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10.1134/S0026893316060212

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title = "Structural studies of chromatin remodeling factors",

abstract = "Changes of chromatin structure require participation of chromatin remodeling factors (CRFs), which are ATP-dependent multisubunit complexes that change the structure of the nucleosome without covalently modifying its components. CRFs act together with other protein factors to regulate the extent of chromatin condensation. Four CRF families are currently distinguished based on their structural and biochemical characteristics: SWI/SNF, ISWI, Mi-2/CHD, and SWR/INO80. X-ray diffraction analysis and electron microscopy are the main methods to obtain structural information about macromolecules. CRFs are difficult to obtain in crystal because of their large sizes and structural heterogeneity, and transmission electron microscopy (TEM) is mostly employed in their structural studies. The review considers all structures obtained for CRFs by TEM and discusses several models of CRF–nucleosome interactions.",

keywords = "Chromatin, Chromatin remodeling factors, Nucleosome, Transcription, Transmission electron microscopy",

author = "Volokh, {O. I.} and Derkacheva, {N. I.} and Studitsky, {V. M.} and Sokolova, {O. S.}",

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year = "2016",

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language = "English",

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TY - JOUR

T1 - Structural studies of chromatin remodeling factors

AU - Volokh, O. I.

AU - Derkacheva, N. I.

AU - Studitsky, V. M.

AU - Sokolova, O. S.

PY - 2016/11

Y1 - 2016/11

N2 - Changes of chromatin structure require participation of chromatin remodeling factors (CRFs), which are ATP-dependent multisubunit complexes that change the structure of the nucleosome without covalently modifying its components. CRFs act together with other protein factors to regulate the extent of chromatin condensation. Four CRF families are currently distinguished based on their structural and biochemical characteristics: SWI/SNF, ISWI, Mi-2/CHD, and SWR/INO80. X-ray diffraction analysis and electron microscopy are the main methods to obtain structural information about macromolecules. CRFs are difficult to obtain in crystal because of their large sizes and structural heterogeneity, and transmission electron microscopy (TEM) is mostly employed in their structural studies. The review considers all structures obtained for CRFs by TEM and discusses several models of CRF–nucleosome interactions.

AB - Changes of chromatin structure require participation of chromatin remodeling factors (CRFs), which are ATP-dependent multisubunit complexes that change the structure of the nucleosome without covalently modifying its components. CRFs act together with other protein factors to regulate the extent of chromatin condensation. Four CRF families are currently distinguished based on their structural and biochemical characteristics: SWI/SNF, ISWI, Mi-2/CHD, and SWR/INO80. X-ray diffraction analysis and electron microscopy are the main methods to obtain structural information about macromolecules. CRFs are difficult to obtain in crystal because of their large sizes and structural heterogeneity, and transmission electron microscopy (TEM) is mostly employed in their structural studies. The review considers all structures obtained for CRFs by TEM and discusses several models of CRF–nucleosome interactions.

KW - Chromatin

KW - Chromatin remodeling factors

KW - Nucleosome

KW - Transcription

KW - Transmission electron microscopy

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DO - 10.1134/S0026893316060212

M3 - Review article

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VL - 50

SP - 812

EP - 822

JO - Molecular Biology

JF - Molecular Biology

IS - 6

ER -

Structural studies of chromatin remodeling factors

Abstract

Keywords

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