Structural and biochemical characterization of the KRLB region in insulin receptor substrate-2

Jinhua Wu, Yolanda D. Tseng, Chong Feng Xu, Thomas A. Neubert, Morris F. White, Stevan R. Hubbard

Research output: Contribution to journalArticlepeer-review

66 Scopus citations

Abstract

Insulin receptor substrates 1 and 2 (IRS1 and -2) are crucial adaptor proteins in mediating the metabolic and mitogenic effects of insulin and insulin-like growth factor 1. These proteins consist of a pleckstrin homology domain, a phosphotyrosine binding domain and a C-terminal region containing numerous sites of tyrosine, serine and threonine phosphorylation. Previous yeast two-hybrid studies identified a region unique to IRS2, termed the kinase regulatory-loop binding (KRLB) region, which interacts with the tyrosine kinase domain of the insulin receptor. Here we present the crystal structure of the insulin receptor kinase in complex with a 15-residue peptide from the KRLB region. In the structure, this segment of IRS2 is bound in the kinase active site with Tyr628 positioned for phosphorylation. Although Tyr628 was phosphorylated by the insulin receptor, its catalytic turnover was poor, resulting in kinase inhibition. Our studies indicate that the KRLB region functions to limit tyrosine phosphorylation of IRS2.

Original languageEnglish
Pages (from-to)251-258
Number of pages8
JournalNature Structural and Molecular Biology
Volume15
Issue number3
DOIs
StatePublished - Mar 2008
Externally publishedYes

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