@inbook{4b9f7c915e2c44bca0666fc681423953,
title = "Site-specific antibody fragment conjugates for targeted imaging",
abstract = "This work details the use of amber suppression-mediated genetic incorporation of unnatural amino acids (UAAs), specifically p-azido-L-phenylalanine (pAzF) and p-acetyl-L-phenylalanine (pAcF), to develop site-specifically labeled antibody Fab fragments. These antibody fragment conjugates represent a novel class of imaging agents with optimal stability, efficacy, and pharmacological properties, which have demonstrated promising potential for probing and understanding the in vivo bio-distributions of protein targets of interest. This chapter provides general guidelines for preparing these Fab conjugates, and details of follow-up bioassays such as single-agent based positron emission tomography (PET) imaging of immune-checkpoint protein PD-L1, and the use of GCN4-mediated switchable antibody conjugates for near-infrared fluorescent imaging of cancer-related biomarkers.",
keywords = "Amino Acids, Antibodies, Immunoglobulin Fab Fragments, Positron-Emission Tomography",
author = "Robert Maloney and Buuh, {Zakey Yusuf} and Yue Zhao and Wang, {Rongsheng E.}",
note = "Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",
year = "2020",
doi = "10.1016/bs.mie.2020.02.023",
language = "English",
isbn = "9780128201459",
volume = "638",
series = "Methods in enzymology",
publisher = "Academic Press Inc.",
pages = "295--320",
editor = "Chenoweth, {David M.}",
booktitle = "Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems",
address = "United States",
}