SHP-1 expression by malignant small B-cell lymphomas reflects the maturation stage of their normal B-cell counterparts

Plamen M. Kossev; Puthiyaveettil N. Raghunath; Adam Bagg; Steven Schuster; John E. Tomaszewski; Mariusz A. Wasik

doi:10.1097/00000478-200107000-00015

SHP-1 expression by malignant small B-cell lymphomas reflects the maturation stage of their normal B-cell counterparts

Plamen M. Kossev, Puthiyaveettil N. Raghunath, Adam Bagg, Steven Schuster, John E. Tomaszewski, Mariusz A. Wasik

University of Pennsylvania

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

SHP-1 is a protein phosphotyrosine phosphatase that plays an important role in modulating intracellular signaling, which regulates cell activation, proliferation, differentiation, and migration. It is a negative regulator of signal transduction induced by a number of cell receptors. Our immunohistochemical examination of paraffin-embedded reactive lymph nodes and lymphoid tissues revealed that B lymphocytes in follicle germinal centers do not express SHP-1. A weak staining of the B cells in the germinal center light zones was detected when an ultrasensitive amplification system was used. In contrast, normal B cells in mantle and marginal zones as well as interfollicular B lymphocytes and plasma cells displayed strong immunoreactivity. This pattern of SHP-1 expression was repeated in small B-cell lymphomas. All cases of mantle cell lymphoma (12 of 12), marginal zone lymphoma (10 of 10), and chronic lymphocytic leukemia/small lymphocytic lymphoma (13 of 13) expressed SHP-1 protein. However, only 1 of 30 cases of grade 1 follicle center cell lymphoma expressed SHP-1. Our observations highlight the biologic functions of SHP-1 and demonstrate that the SHP-1 expression pattern by small B-cell lymphomas reflects the maturation stage of their normal cell counterparts. These results indicate that determination of SHP-1 expression may help in the differential diagnosis of small B-cell lymphomas.

Original language	English
Pages (from-to)	949-955
Number of pages	7
Journal	American Journal of Surgical Pathology
Volume	25
Issue number	7
DOIs	https://doi.org/10.1097/00000478-200107000-00015
State	Published - 2001

Keywords

B-Lymphocytes/physiology
Cellular Senescence
Humans
Immunohistochemistry/methods
Intracellular Signaling Peptides and Proteins
Leukemia, Lymphocytic, Chronic, B-Cell/metabolism
Lymph Nodes/metabolism
Lymphoid Tissue/metabolism
Lymphoma, B-Cell/metabolism
Protein Tyrosine Phosphatase, Non-Receptor Type 6
Protein Tyrosine Phosphatases/metabolism
Reference Values
Staining and Labeling/methods

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10.1097/00000478-200107000-00015

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title = "SHP-1 expression by malignant small B-cell lymphomas reflects the maturation stage of their normal B-cell counterparts",

abstract = "SHP-1 is a protein phosphotyrosine phosphatase that plays an important role in modulating intracellular signaling, which regulates cell activation, proliferation, differentiation, and migration. It is a negative regulator of signal transduction induced by a number of cell receptors. Our immunohistochemical examination of paraffin-embedded reactive lymph nodes and lymphoid tissues revealed that B lymphocytes in follicle germinal centers do not express SHP-1. A weak staining of the B cells in the germinal center light zones was detected when an ultrasensitive amplification system was used. In contrast, normal B cells in mantle and marginal zones as well as interfollicular B lymphocytes and plasma cells displayed strong immunoreactivity. This pattern of SHP-1 expression was repeated in small B-cell lymphomas. All cases of mantle cell lymphoma (12 of 12), marginal zone lymphoma (10 of 10), and chronic lymphocytic leukemia/small lymphocytic lymphoma (13 of 13) expressed SHP-1 protein. However, only 1 of 30 cases of grade 1 follicle center cell lymphoma expressed SHP-1. Our observations highlight the biologic functions of SHP-1 and demonstrate that the SHP-1 expression pattern by small B-cell lymphomas reflects the maturation stage of their normal cell counterparts. These results indicate that determination of SHP-1 expression may help in the differential diagnosis of small B-cell lymphomas.",

keywords = "B-Lymphocytes/physiology, Cellular Senescence, Humans, Immunohistochemistry/methods, Intracellular Signaling Peptides and Proteins, Leukemia, Lymphocytic, Chronic, B-Cell/metabolism, Lymph Nodes/metabolism, Lymphoid Tissue/metabolism, Lymphoma, B-Cell/metabolism, Protein Tyrosine Phosphatase, Non-Receptor Type 6, Protein Tyrosine Phosphatases/metabolism, Reference Values, Staining and Labeling/methods",

author = "Kossev, {Plamen M.} and Raghunath, {Puthiyaveettil N.} and Adam Bagg and Steven Schuster and Tomaszewski, {John E.} and Wasik, {Mariusz A.}",

year = "2001",

doi = "10.1097/00000478-200107000-00015",

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T1 - SHP-1 expression by malignant small B-cell lymphomas reflects the maturation stage of their normal B-cell counterparts

AU - Kossev, Plamen M.

AU - Raghunath, Puthiyaveettil N.

AU - Bagg, Adam

AU - Schuster, Steven

AU - Tomaszewski, John E.

AU - Wasik, Mariusz A.

PY - 2001

Y1 - 2001

N2 - SHP-1 is a protein phosphotyrosine phosphatase that plays an important role in modulating intracellular signaling, which regulates cell activation, proliferation, differentiation, and migration. It is a negative regulator of signal transduction induced by a number of cell receptors. Our immunohistochemical examination of paraffin-embedded reactive lymph nodes and lymphoid tissues revealed that B lymphocytes in follicle germinal centers do not express SHP-1. A weak staining of the B cells in the germinal center light zones was detected when an ultrasensitive amplification system was used. In contrast, normal B cells in mantle and marginal zones as well as interfollicular B lymphocytes and plasma cells displayed strong immunoreactivity. This pattern of SHP-1 expression was repeated in small B-cell lymphomas. All cases of mantle cell lymphoma (12 of 12), marginal zone lymphoma (10 of 10), and chronic lymphocytic leukemia/small lymphocytic lymphoma (13 of 13) expressed SHP-1 protein. However, only 1 of 30 cases of grade 1 follicle center cell lymphoma expressed SHP-1. Our observations highlight the biologic functions of SHP-1 and demonstrate that the SHP-1 expression pattern by small B-cell lymphomas reflects the maturation stage of their normal cell counterparts. These results indicate that determination of SHP-1 expression may help in the differential diagnosis of small B-cell lymphomas.

AB - SHP-1 is a protein phosphotyrosine phosphatase that plays an important role in modulating intracellular signaling, which regulates cell activation, proliferation, differentiation, and migration. It is a negative regulator of signal transduction induced by a number of cell receptors. Our immunohistochemical examination of paraffin-embedded reactive lymph nodes and lymphoid tissues revealed that B lymphocytes in follicle germinal centers do not express SHP-1. A weak staining of the B cells in the germinal center light zones was detected when an ultrasensitive amplification system was used. In contrast, normal B cells in mantle and marginal zones as well as interfollicular B lymphocytes and plasma cells displayed strong immunoreactivity. This pattern of SHP-1 expression was repeated in small B-cell lymphomas. All cases of mantle cell lymphoma (12 of 12), marginal zone lymphoma (10 of 10), and chronic lymphocytic leukemia/small lymphocytic lymphoma (13 of 13) expressed SHP-1 protein. However, only 1 of 30 cases of grade 1 follicle center cell lymphoma expressed SHP-1. Our observations highlight the biologic functions of SHP-1 and demonstrate that the SHP-1 expression pattern by small B-cell lymphomas reflects the maturation stage of their normal cell counterparts. These results indicate that determination of SHP-1 expression may help in the differential diagnosis of small B-cell lymphomas.

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KW - Immunohistochemistry/methods

KW - Intracellular Signaling Peptides and Proteins

KW - Leukemia, Lymphocytic, Chronic, B-Cell/metabolism

KW - Lymph Nodes/metabolism

KW - Lymphoid Tissue/metabolism

KW - Lymphoma, B-Cell/metabolism

KW - Protein Tyrosine Phosphatase, Non-Receptor Type 6

KW - Protein Tyrosine Phosphatases/metabolism

KW - Reference Values

KW - Staining and Labeling/methods

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SN - 0147-5185

VL - 25

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EP - 955

JO - American Journal of Surgical Pathology

JF - American Journal of Surgical Pathology

IS - 7

ER -

SHP-1 expression by malignant small B-cell lymphomas reflects the maturation stage of their normal B-cell counterparts

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