Serum apolipoprotein A-1 quantification by LC-MS with a SILAC internal standard reveals reduced levels in smokers

Qingqing Wang; Suhong Zhang; Lili Guo; Christine M. Busch; Wenying Jian; Naidong Weng; Nathaniel W. Snyder; Kannan Rangiah; Clementina Mesaros; Ian A. Blair

doi:10.4155/bio.15.195

Serum apolipoprotein A-1 quantification by LC-MS with a SILAC internal standard reveals reduced levels in smokers

Qingqing Wang, Suhong Zhang, Lili Guo, Christine M. Busch, Wenying Jian, Naidong Weng, Nathaniel W. Snyder, Kannan Rangiah, Clementina Mesaros, Ian A. Blair

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Background: Absolute quantification of protein biomarkers such as serum apolipoprotein A1 by both immunoassays and LC-MS can provide misleading results. Results: Recombinant ApoA-1 internal standard was prepared using stable isotope labeling by amino acids in cell culture with [C₆N₂]-lysine and [C₉N₁]-tyrosine in human cells. A stable isotope dilution LC-MS method for serum ApoA-1 was validated and levels analyzed for 50 nonsmokers and 50 smokers. Conclusion: The concentration of ApoA-1 in nonsmokers was 169.4 mg/dl with an 18.4% reduction to 138.2 mg/dl in smokers. The validated assay will have clinical utility for assessing effects of smoking cessation and therapeutic or dietary interventions in high-risk populations.

Original language	English
Pages (from-to)	2895-2911
Number of pages	17
Journal	Bioanalysis
Volume	7
Issue number	22
DOIs	https://doi.org/10.4155/bio.15.195
State	Published - Nov 2015
Externally published	Yes

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title = "Serum apolipoprotein A-1 quantification by LC-MS with a SILAC internal standard reveals reduced levels in smokers",

abstract = "Background: Absolute quantification of protein biomarkers such as serum apolipoprotein A1 by both immunoassays and LC-MS can provide misleading results. Results: Recombinant ApoA-1 internal standard was prepared using stable isotope labeling by amino acids in cell culture with [C6N2]-lysine and [C9N1]-tyrosine in human cells. A stable isotope dilution LC-MS method for serum ApoA-1 was validated and levels analyzed for 50 nonsmokers and 50 smokers. Conclusion: The concentration of ApoA-1 in nonsmokers was 169.4 mg/dl with an 18.4% reduction to 138.2 mg/dl in smokers. The validated assay will have clinical utility for assessing effects of smoking cessation and therapeutic or dietary interventions in high-risk populations.",

author = "Qingqing Wang and Suhong Zhang and Lili Guo and Busch, {Christine M.} and Wenying Jian and Naidong Weng and Snyder, {Nathaniel W.} and Kannan Rangiah and Clementina Mesaros and Blair, {Ian A.}",

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year = "2015",

month = nov,

doi = "10.4155/bio.15.195",

language = "English",

volume = "7",

pages = "2895--2911",

journal = "Bioanalysis",

issn = "1757-6180",

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TY - JOUR

T1 - Serum apolipoprotein A-1 quantification by LC-MS with a SILAC internal standard reveals reduced levels in smokers

AU - Wang, Qingqing

AU - Zhang, Suhong

AU - Guo, Lili

AU - Busch, Christine M.

AU - Jian, Wenying

AU - Weng, Naidong

AU - Snyder, Nathaniel W.

AU - Rangiah, Kannan

AU - Mesaros, Clementina

AU - Blair, Ian A.

PY - 2015/11

Y1 - 2015/11

N2 - Background: Absolute quantification of protein biomarkers such as serum apolipoprotein A1 by both immunoassays and LC-MS can provide misleading results. Results: Recombinant ApoA-1 internal standard was prepared using stable isotope labeling by amino acids in cell culture with [C6N2]-lysine and [C9N1]-tyrosine in human cells. A stable isotope dilution LC-MS method for serum ApoA-1 was validated and levels analyzed for 50 nonsmokers and 50 smokers. Conclusion: The concentration of ApoA-1 in nonsmokers was 169.4 mg/dl with an 18.4% reduction to 138.2 mg/dl in smokers. The validated assay will have clinical utility for assessing effects of smoking cessation and therapeutic or dietary interventions in high-risk populations.

AB - Background: Absolute quantification of protein biomarkers such as serum apolipoprotein A1 by both immunoassays and LC-MS can provide misleading results. Results: Recombinant ApoA-1 internal standard was prepared using stable isotope labeling by amino acids in cell culture with [C6N2]-lysine and [C9N1]-tyrosine in human cells. A stable isotope dilution LC-MS method for serum ApoA-1 was validated and levels analyzed for 50 nonsmokers and 50 smokers. Conclusion: The concentration of ApoA-1 in nonsmokers was 169.4 mg/dl with an 18.4% reduction to 138.2 mg/dl in smokers. The validated assay will have clinical utility for assessing effects of smoking cessation and therapeutic or dietary interventions in high-risk populations.

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ER -

Serum apolipoprotein A-1 quantification by LC-MS with a SILAC internal standard reveals reduced levels in smokers

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