RNA interference: A potential strategy for isoform-specific phosphatidylinositol 3-kinase targeted therapy in ovarian cancer

Lin Zhang; Nuo Yang; Shun Liang; Andrea Barchetti; Cristina Vezzani; Jia Huang; Ann O'Brien-Jenkins; Stephen C. Rubin; George Coukos

doi:10.4161/cbt.3.12.1388

RNA interference: A potential strategy for isoform-specific phosphatidylinositol 3-kinase targeted therapy in ovarian cancer

Lin Zhang
, Nuo Yang
, Shun Liang
, Andrea Barchetti
, Cristina Vezzani
, Jia Huang
, Ann O'Brien-Jenkins
, Stephen C. Rubin
, George Coukos

Surgery

University of Pennsylvania

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Phosphatidylinositol 3-kinase (PI3-kinase) is a novel intracellular transducer involved in a wide range of cancer-associated signaling pathways, which comprises various isoforms and splice variants with distinct biologic activities and clinical implications. Especially, the class Ia PI3-kinase 110 kD catalytic subunit alpha (PIK3CA) is the most important isoform in tumorigenesis and possibly, tumor angiogenesis. Several strategies have been developed to block PI3-kinase for cancer therapy; however, the approach to target specific PI3-kinase isoform has not been explored to date. In the present study, we show that RNA interference (RNAi) through small interfering (siRNA) sequences targeting PIK3CA has potential applications in isoform-specific "knock-down" of PI3-kinase. This strategy provides a novel tool to study the function of various PI3-kinase isoforms and may contribute to isoform- specific targeting of PI3-kinase in human cancer.

Original language	English
Pages (from-to)	1283-1289
Number of pages	7
Journal	Cancer Biology and Therapy
Volume	3
Issue number	12
DOIs	https://doi.org/10.4161/cbt.3.12.1388
State	Published - Dec 2004

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Animals
Biomarkers, Tumor/metabolism
Blotting, Western
Cell Proliferation
Class I Phosphatidylinositol 3-Kinases
Female
Genetic Vectors
Humans
Mice
Ovarian Neoplasms/genetics
Phosphatidylinositol 3-Kinases/genetics
Phosphoinositide-3 Kinase Inhibitors
Protein Isoforms
RNA Interference
RNA, Small Interfering/pharmacology
Tumor Cells, Cultured

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10.4161/cbt.3.12.1388

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title = "RNA interference: A potential strategy for isoform-specific phosphatidylinositol 3-kinase targeted therapy in ovarian cancer",

abstract = "Phosphatidylinositol 3-kinase (PI3-kinase) is a novel intracellular transducer involved in a wide range of cancer-associated signaling pathways, which comprises various isoforms and splice variants with distinct biologic activities and clinical implications. Especially, the class Ia PI3-kinase 110 kD catalytic subunit alpha (PIK3CA) is the most important isoform in tumorigenesis and possibly, tumor angiogenesis. Several strategies have been developed to block PI3-kinase for cancer therapy; however, the approach to target specific PI3-kinase isoform has not been explored to date. In the present study, we show that RNA interference (RNAi) through small interfering (siRNA) sequences targeting PIK3CA has potential applications in isoform-specific {"}knock-down{"} of PI3-kinase. This strategy provides a novel tool to study the function of various PI3-kinase isoforms and may contribute to isoform- specific targeting of PI3-kinase in human cancer.",

keywords = "Animals, Biomarkers, Tumor/metabolism, Blotting, Western, Cell Proliferation, Class I Phosphatidylinositol 3-Kinases, Female, Genetic Vectors, Humans, Mice, Ovarian Neoplasms/genetics, Phosphatidylinositol 3-Kinases/genetics, Phosphoinositide-3 Kinase Inhibitors, Protein Isoforms, RNA Interference, RNA, Small Interfering/pharmacology, Tumor Cells, Cultured",

author = "Lin Zhang and Nuo Yang and Shun Liang and Andrea Barchetti and Cristina Vezzani and Jia Huang and Ann O'Brien-Jenkins and Rubin, \{Stephen C.\} and George Coukos",

year = "2004",

month = dec,

doi = "10.4161/cbt.3.12.1388",

language = "English",

volume = "3",

pages = "1283--1289",

journal = "Cancer Biology and Therapy",

issn = "1538-4047",

publisher = "Taylor and Francis Ltd.",

number = "12",

}

TY - JOUR

T1 - RNA interference

T2 - A potential strategy for isoform-specific phosphatidylinositol 3-kinase targeted therapy in ovarian cancer

AU - Zhang, Lin

AU - Yang, Nuo

AU - Liang, Shun

AU - Barchetti, Andrea

AU - Vezzani, Cristina

AU - Huang, Jia

AU - O'Brien-Jenkins, Ann

AU - Rubin, Stephen C.

AU - Coukos, George

PY - 2004/12

Y1 - 2004/12

N2 - Phosphatidylinositol 3-kinase (PI3-kinase) is a novel intracellular transducer involved in a wide range of cancer-associated signaling pathways, which comprises various isoforms and splice variants with distinct biologic activities and clinical implications. Especially, the class Ia PI3-kinase 110 kD catalytic subunit alpha (PIK3CA) is the most important isoform in tumorigenesis and possibly, tumor angiogenesis. Several strategies have been developed to block PI3-kinase for cancer therapy; however, the approach to target specific PI3-kinase isoform has not been explored to date. In the present study, we show that RNA interference (RNAi) through small interfering (siRNA) sequences targeting PIK3CA has potential applications in isoform-specific "knock-down" of PI3-kinase. This strategy provides a novel tool to study the function of various PI3-kinase isoforms and may contribute to isoform- specific targeting of PI3-kinase in human cancer.

AB - Phosphatidylinositol 3-kinase (PI3-kinase) is a novel intracellular transducer involved in a wide range of cancer-associated signaling pathways, which comprises various isoforms and splice variants with distinct biologic activities and clinical implications. Especially, the class Ia PI3-kinase 110 kD catalytic subunit alpha (PIK3CA) is the most important isoform in tumorigenesis and possibly, tumor angiogenesis. Several strategies have been developed to block PI3-kinase for cancer therapy; however, the approach to target specific PI3-kinase isoform has not been explored to date. In the present study, we show that RNA interference (RNAi) through small interfering (siRNA) sequences targeting PIK3CA has potential applications in isoform-specific "knock-down" of PI3-kinase. This strategy provides a novel tool to study the function of various PI3-kinase isoforms and may contribute to isoform- specific targeting of PI3-kinase in human cancer.

KW - Animals

KW - Biomarkers, Tumor/metabolism

KW - Blotting, Western

KW - Cell Proliferation

KW - Class I Phosphatidylinositol 3-Kinases

KW - Female

KW - Genetic Vectors

KW - Humans

KW - Mice

KW - Ovarian Neoplasms/genetics

KW - Phosphatidylinositol 3-Kinases/genetics

KW - Phosphoinositide-3 Kinase Inhibitors

KW - Protein Isoforms

KW - RNA Interference

KW - RNA, Small Interfering/pharmacology

KW - Tumor Cells, Cultured

UR - https://www.scopus.com/pages/publications/22344446375

U2 - 10.4161/cbt.3.12.1388

DO - 10.4161/cbt.3.12.1388

M3 - Article

C2 - 15662137

SN - 1538-4047

VL - 3

SP - 1283

EP - 1289

JO - Cancer Biology and Therapy

JF - Cancer Biology and Therapy

IS - 12

ER -

RNA interference: A potential strategy for isoform-specific phosphatidylinositol 3-kinase targeted therapy in ovarian cancer

Abstract

UN SDGs

Keywords

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