Replication-defective missense mutations within the terminal protein and spacer/intron regions of the polymerase gene of human hepatitis B virus

Ahmed F. Faruqi; Susanta Roychoudhury; Richard Greenberg; Jonathan Israel; Chiaho Shih

doi:10.1016/0042-6822(91)91007-4

Replication-defective missense mutations within the terminal protein and spacer/intron regions of the polymerase gene of human hepatitis B virus

Ahmed F. Faruqi, Susanta Roychoudhury, Richard Greenberg, Jonathan Israel, Chiaho Shih

University of Pennsylvania

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

We have analyzed 11 independent mutations located at various domains of the polymerase gene (pol) of human hepatitis B virus. Surprisingly, one of the two missense mutants within the spacer/intron region appears to be lethal. This result further defines the N-terminal limit of the reverse transcriptase domain. Alternatively, it suggests the potential existence of a novel domain with an unknown function. Two missense mutations within the terminal protein (TP) domain appear to be replication-defective as well, suggesting a functionally essential role of the TP domain in DNA replication.

Original language	English
Pages (from-to)	764-768
Number of pages	5
Journal	Virology
Volume	183
Issue number	2
DOIs	https://doi.org/10.1016/0042-6822(91)91007-4
State	Published - Aug 1991

Keywords

Blotting, Northern
Blotting, Southern
Cell Line
DNA, Ribosomal
DNA, Viral
Genes, pol
Hepatitis B virus/genetics
Humans
Introns
Mutation
Transcription, Genetic
Virus Replication/genetics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0042-6822(91)91007-4

Cite this

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title = "Replication-defective missense mutations within the terminal protein and spacer/intron regions of the polymerase gene of human hepatitis B virus",

abstract = "We have analyzed 11 independent mutations located at various domains of the polymerase gene (pol) of human hepatitis B virus. Surprisingly, one of the two missense mutants within the spacer/intron region appears to be lethal. This result further defines the N-terminal limit of the reverse transcriptase domain. Alternatively, it suggests the potential existence of a novel domain with an unknown function. Two missense mutations within the terminal protein (TP) domain appear to be replication-defective as well, suggesting a functionally essential role of the TP domain in DNA replication.",

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author = "Faruqi, {Ahmed F.} and Susanta Roychoudhury and Richard Greenberg and Jonathan Israel and Chiaho Shih",

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T1 - Replication-defective missense mutations within the terminal protein and spacer/intron regions of the polymerase gene of human hepatitis B virus

AU - Faruqi, Ahmed F.

AU - Roychoudhury, Susanta

AU - Greenberg, Richard

AU - Israel, Jonathan

AU - Shih, Chiaho

PY - 1991/8

Y1 - 1991/8

N2 - We have analyzed 11 independent mutations located at various domains of the polymerase gene (pol) of human hepatitis B virus. Surprisingly, one of the two missense mutants within the spacer/intron region appears to be lethal. This result further defines the N-terminal limit of the reverse transcriptase domain. Alternatively, it suggests the potential existence of a novel domain with an unknown function. Two missense mutations within the terminal protein (TP) domain appear to be replication-defective as well, suggesting a functionally essential role of the TP domain in DNA replication.

AB - We have analyzed 11 independent mutations located at various domains of the polymerase gene (pol) of human hepatitis B virus. Surprisingly, one of the two missense mutants within the spacer/intron region appears to be lethal. This result further defines the N-terminal limit of the reverse transcriptase domain. Alternatively, it suggests the potential existence of a novel domain with an unknown function. Two missense mutations within the terminal protein (TP) domain appear to be replication-defective as well, suggesting a functionally essential role of the TP domain in DNA replication.

KW - Blotting, Northern

KW - Blotting, Southern

KW - Cell Line

KW - DNA, Ribosomal

KW - DNA, Viral

KW - Genes, pol

KW - Hepatitis B virus/genetics

KW - Humans

KW - Introns

KW - Mutation

KW - Transcription, Genetic

KW - Virus Replication/genetics

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DO - 10.1016/0042-6822(91)91007-4

M3 - Article

C2 - 1853574

AN - SCOPUS:0025848284

SN - 0042-6822

VL - 183

SP - 764

EP - 768

JO - Virology

JF - Virology

IS - 2

ER -

Replication-defective missense mutations within the terminal protein and spacer/intron regions of the polymerase gene of human hepatitis B virus

Abstract

Keywords

UN SDGs

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