Abstract
The Rac guanosine 5′-triphosphate (GTP)-binding proteins regulate oxidant production by phagocytic leukocytes. Two Ste20-related p21-activated kinases (PAKs) were identified as targets of Rac in human neutrophils. Activity of the ∼65- and ∼68-kilodalton PAKs was rapidly stimulated by chemoattractants acting through pertussis toxin-sensitive heterotrimeric GTP-binding proteins (G proteins). Native and recombinant PAKs phosphorylated the p47phox reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase component in a Rac-GTP-dependent manner. The action of PAKs during phagocyte activation by G protein-coupled pathways may contribute to regulation of NADPH oxidase activity.
Original language | English |
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Pages (from-to) | 221-223 |
Number of pages | 3 |
Journal | Science |
Volume | 269 |
Issue number | 5221 |
DOIs | |
State | Published - 1995 |
Keywords
- Adenosine Triphosphate/metabolism
- Enzyme Activation
- GTP-Binding Proteins/metabolism
- Guanosine Triphosphate/metabolism
- Humans
- N-Formylmethionine Leucyl-Phenylalanine/pharmacology
- NADH, NADPH Oxidoreductases/metabolism
- NADPH Dehydrogenase/metabolism
- NADPH Oxidases
- Neutrophil Activation
- Neutrophils/enzymology
- Pertussis Toxin
- Phosphoproteins/metabolism
- Phosphorylation
- Protein Serine-Threonine Kinases/metabolism
- Recombinant Proteins/metabolism
- Tetradecanoylphorbol Acetate/pharmacology
- Virulence Factors, Bordetella/pharmacology
- p21-Activated Kinases
- rac GTP-Binding Proteins