Purification of differentiated tumor cells from medulloblastoma for transplantation into mouse cerebellum

Yijun Yang; Yanghui Qu; Yan Cheng; Duancheng Guo; Qianhai Fan; Zeng jie Yang

doi:10.1016/j.xpro.2021.100409

Purification of differentiated tumor cells from medulloblastoma for transplantation into mouse cerebellum

Yijun Yang, Yanghui Qu, Yan Cheng, Duancheng Guo, Qianhai Fan, Zeng jie Yang

Research output: Contribution to journal › Article › peer-review

Abstract

This protocol provides the procedures for isolating differentiated tumor cells from medulloblastoma (MB) in mice. Procedures for transplantation into cerebella are also included to examine the tumorigenesis of differentiated MB cells. This protocol outlines the detailed steps required for (1) isolation of tumor cells from mouse MB, (2) purification of differentiated tumor cells by fluorescence-activated cell sorting, and (3) transplantation of tumor cells into cerebella. This protocol is useful to purify differentiated tumor cells for investigating mechanisms underlying MB progression.

Original language	English
Article number	100409
Pages (from-to)	100409
Journal	STAR Protocols
Volume	2
Issue number	2
DOIs	https://doi.org/10.1016/j.xpro.2021.100409
State	Published - Jun 18 2021

Keywords

Animals
Cell Culture Techniques/methods
Cell Differentiation
Cell Separation/methods
Cell Transplantation
Cerebellar Neoplasms/pathology
Cerebellum/surgery
Medulloblastoma/pathology
Mice
Tumor Cells, Cultured/cytology

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10.1016/j.xpro.2021.100409

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title = "Purification of differentiated tumor cells from medulloblastoma for transplantation into mouse cerebellum",

abstract = "This protocol provides the procedures for isolating differentiated tumor cells from medulloblastoma (MB) in mice. Procedures for transplantation into cerebella are also included to examine the tumorigenesis of differentiated MB cells. This protocol outlines the detailed steps required for (1) isolation of tumor cells from mouse MB, (2) purification of differentiated tumor cells by fluorescence-activated cell sorting, and (3) transplantation of tumor cells into cerebella. This protocol is useful to purify differentiated tumor cells for investigating mechanisms underlying MB progression.",

keywords = "Animals, Cell Culture Techniques/methods, Cell Differentiation, Cell Separation/methods, Cell Transplantation, Cerebellar Neoplasms/pathology, Cerebellum/surgery, Medulloblastoma/pathology, Mice, Tumor Cells, Cultured/cytology",

author = "Yijun Yang and Yanghui Qu and Yan Cheng and Duancheng Guo and Qianhai Fan and Yang, {Zeng jie}",

note = "Publisher Copyright: {\textcopyright} 2021 The Author(s)",

year = "2021",

month = jun,

day = "18",

doi = "10.1016/j.xpro.2021.100409",

language = "English",

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journal = "STAR Protocols",

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T1 - Purification of differentiated tumor cells from medulloblastoma for transplantation into mouse cerebellum

AU - Yang, Yijun

AU - Qu, Yanghui

AU - Cheng, Yan

AU - Guo, Duancheng

AU - Fan, Qianhai

AU - Yang, Zeng jie

PY - 2021/6/18

Y1 - 2021/6/18

N2 - This protocol provides the procedures for isolating differentiated tumor cells from medulloblastoma (MB) in mice. Procedures for transplantation into cerebella are also included to examine the tumorigenesis of differentiated MB cells. This protocol outlines the detailed steps required for (1) isolation of tumor cells from mouse MB, (2) purification of differentiated tumor cells by fluorescence-activated cell sorting, and (3) transplantation of tumor cells into cerebella. This protocol is useful to purify differentiated tumor cells for investigating mechanisms underlying MB progression.

AB - This protocol provides the procedures for isolating differentiated tumor cells from medulloblastoma (MB) in mice. Procedures for transplantation into cerebella are also included to examine the tumorigenesis of differentiated MB cells. This protocol outlines the detailed steps required for (1) isolation of tumor cells from mouse MB, (2) purification of differentiated tumor cells by fluorescence-activated cell sorting, and (3) transplantation of tumor cells into cerebella. This protocol is useful to purify differentiated tumor cells for investigating mechanisms underlying MB progression.

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KW - Cell Culture Techniques/methods

KW - Cell Differentiation

KW - Cell Separation/methods

KW - Cell Transplantation

KW - Cerebellar Neoplasms/pathology

KW - Cerebellum/surgery

KW - Medulloblastoma/pathology

KW - Mice

KW - Tumor Cells, Cultured/cytology

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DO - 10.1016/j.xpro.2021.100409

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SP - 100409

JO - STAR Protocols

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Purification of differentiated tumor cells from medulloblastoma for transplantation into mouse cerebellum

Abstract

Keywords

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Cell Culture Facility

Cell Sorting Facility

Histopathology Facility

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Purification of differentiated tumor cells from medulloblastoma for transplantation into mouse cerebellum

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Equipment

Cell Culture Facility

Cell Sorting Facility

Histopathology Facility

Cite this