Protocol to monitor cardiovascular effects of triiodothyronine in tumor-bearing mice via non-invasive electrocardiogram recordings

Silvia Anahi Valdés-Rives; Yijun Yang; Zeng jie Yang

doi:10.1016/j.xpro.2025.103900

Protocol to monitor cardiovascular effects of triiodothyronine in tumor-bearing mice via non-invasive electrocardiogram recordings

Silvia Anahi Valdés-Rives
, Yijun Yang
, Zeng jie Yang

Temple University
Fox Chase Cancer Center

Research output: Contribution to journal › Article › peer-review

Abstract

Understanding the cardiovascular side effects of cancer therapies is essential in preclinical research. We present a step-by-step protocol to evaluate cardiovascular function in triiodothyronine-treated, tumor-bearing mice using non-invasive electrocardiogram (ECG) recordings. The steps involve acclimating awake mice to recording towers, obtaining ECG traces without anesthesia, and assessing cardiac parameters. This approach enables reliable monitoring of treatment-induced cardiac effects and supports cardiovascular safety evaluation during preclinical cancer drug development. For complete details on the use and execution of this protocol, please refer to Yang et al.¹

Original language	English
Article number	103900
Pages (from-to)	103900
Journal	Star Protocols
Volume	6
Issue number	3
Early online date	Jun 20 2025
DOIs	https://doi.org/10.1016/j.xpro.2025.103900
State	Published - Sep 19 2025

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Cancer
Cell Biology
Clinical Protocol

Access to Document

10.1016/j.xpro.2025.103900

Cite this

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title = "Protocol to monitor cardiovascular effects of triiodothyronine in tumor-bearing mice via non-invasive electrocardiogram recordings",

abstract = "Understanding the cardiovascular side effects of cancer therapies is essential in preclinical research. We present a step-by-step protocol to evaluate cardiovascular function in triiodothyronine-treated, tumor-bearing mice using non-invasive electrocardiogram (ECG) recordings. The steps involve acclimating awake mice to recording towers, obtaining ECG traces without anesthesia, and assessing cardiac parameters. This approach enables reliable monitoring of treatment-induced cardiac effects and supports cardiovascular safety evaluation during preclinical cancer drug development. For complete details on the use and execution of this protocol, please refer to Yang et al.1",

keywords = "Cancer, Cell Biology, Clinical Protocol",

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T1 - Protocol to monitor cardiovascular effects of triiodothyronine in tumor-bearing mice via non-invasive electrocardiogram recordings

AU - Valdés-Rives, Silvia Anahi

AU - Yang, Yijun

AU - Yang, Zeng jie

PY - 2025/9/19

Y1 - 2025/9/19

N2 - Understanding the cardiovascular side effects of cancer therapies is essential in preclinical research. We present a step-by-step protocol to evaluate cardiovascular function in triiodothyronine-treated, tumor-bearing mice using non-invasive electrocardiogram (ECG) recordings. The steps involve acclimating awake mice to recording towers, obtaining ECG traces without anesthesia, and assessing cardiac parameters. This approach enables reliable monitoring of treatment-induced cardiac effects and supports cardiovascular safety evaluation during preclinical cancer drug development. For complete details on the use and execution of this protocol, please refer to Yang et al.1

AB - Understanding the cardiovascular side effects of cancer therapies is essential in preclinical research. We present a step-by-step protocol to evaluate cardiovascular function in triiodothyronine-treated, tumor-bearing mice using non-invasive electrocardiogram (ECG) recordings. The steps involve acclimating awake mice to recording towers, obtaining ECG traces without anesthesia, and assessing cardiac parameters. This approach enables reliable monitoring of treatment-induced cardiac effects and supports cardiovascular safety evaluation during preclinical cancer drug development. For complete details on the use and execution of this protocol, please refer to Yang et al.1

KW - Cancer

KW - Cell Biology

KW - Clinical Protocol

UR - https://www.scopus.com/pages/publications/105008531653

U2 - 10.1016/j.xpro.2025.103900

DO - 10.1016/j.xpro.2025.103900

M3 - Article

C2 - 40543037

AN - SCOPUS:105008531653

SN - 2666-1667

VL - 6

SP - 103900

JO - Star Protocols

JF - Star Protocols

IS - 3

M1 - 103900

ER -

Protocol to monitor cardiovascular effects of triiodothyronine in tumor-bearing mice via non-invasive electrocardiogram recordings

Abstract

UN SDGs

Keywords

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