Preparation of extracellular matrices produced by cultured and primary fibroblasts.

Dorothy A. Beacham; Michael D. Amatangelo; Edna Cukierman

doi:10.1002/0471143030.cb1009s33

Preparation of extracellular matrices produced by cultured and primary fibroblasts.

Dorothy A. Beacham, Michael D. Amatangelo, Edna Cukierman

Fox Chase Cancer Center

Research output: Contribution to journal › Article › peer-review

69 Scopus citations

Abstract

Fibroblasts secrete and organize extracellular matrix (ECM), which provides structural support for their adhesion, migration, and tissue organization, besides regulating cellular functions such as growth and survival. Cell-to-matrix interactions are vital for vertebrate development. Disorders in these processes have been associated with fibrosis, developmental malformations, cancer, and other diseases. This unit describes a method for preparing a three-dimensional matrix derived from NIH-3T3 cells; the matrix is three-dimensions, cell and debris free, and attached to a two-dimensional culture surface. Cell adhesion and spreading are normal on these matrices. This matrix can also be compressed into a two-dimensional matrix and solubilized to study the matrix biochemically.

Original language	English
Pages (from-to)	Unit 10.9
Journal	Current Protocols in Cell Biology
Volume	Chapter 10
DOIs	https://doi.org/10.1002/0471143030.cb1009s33
State	Published - Jan 2007

Keywords

3T3 Cells
Animals
Cell Culture Techniques/methods
Cells, Cultured
Extracellular Matrix
Fibroblasts/metabolism
Mice

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/0471143030.cb1009s33

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AU - Amatangelo, Michael D.

AU - Cukierman, Edna

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AB - Fibroblasts secrete and organize extracellular matrix (ECM), which provides structural support for their adhesion, migration, and tissue organization, besides regulating cellular functions such as growth and survival. Cell-to-matrix interactions are vital for vertebrate development. Disorders in these processes have been associated with fibrosis, developmental malformations, cancer, and other diseases. This unit describes a method for preparing a three-dimensional matrix derived from NIH-3T3 cells; the matrix is three-dimensions, cell and debris free, and attached to a two-dimensional culture surface. Cell adhesion and spreading are normal on these matrices. This matrix can also be compressed into a two-dimensional matrix and solubilized to study the matrix biochemically.

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KW - Animals

KW - Cell Culture Techniques/methods

KW - Cells, Cultured

KW - Extracellular Matrix

KW - Fibroblasts/metabolism

KW - Mice

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Preparation of extracellular matrices produced by cultured and primary fibroblasts.

Abstract

Keywords

UN SDGs

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