TY - JOUR
T1 - Preparation and analysis of uniquely positioned mononucleosomes.
AU - Gaykalova, Daria A.
AU - Kulaeva, Olga I.
AU - Bondarenko, Vladimir A.
AU - Studitsky, Vasily M.
PY - 2009
Y1 - 2009
N2 - Short DNA fragments containing single, uniquely positioned nucleosome cores have been extensively employed as simple model experimental systems for analysis of many intranuclear processes, including binding of proteins to nucleosomes, transcription, DNA repair and ATP-dependent chromatin remodeling. In many cases such simple model templates faithfully recapitulate numerous important aspects of these processes. Here we describe several recently developed procedures for obtaining and analysis of mononucleosomes that are uniquely positioned on 150-600 bp DNA fragments.
AB - Short DNA fragments containing single, uniquely positioned nucleosome cores have been extensively employed as simple model experimental systems for analysis of many intranuclear processes, including binding of proteins to nucleosomes, transcription, DNA repair and ATP-dependent chromatin remodeling. In many cases such simple model templates faithfully recapitulate numerous important aspects of these processes. Here we describe several recently developed procedures for obtaining and analysis of mononucleosomes that are uniquely positioned on 150-600 bp DNA fragments.
UR - http://www.scopus.com/inward/record.url?scp=79955995191&partnerID=8YFLogxK
U2 - 10.1007/978-1-59745-190-1_8
DO - 10.1007/978-1-59745-190-1_8
M3 - Article
C2 - 19381918
AN - SCOPUS:79955995191
SN - 1064-3745
VL - 523
SP - 109
EP - 123
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -