Preparation and analysis of positioned mononucleosomes

Olga I. Kulaeva, Vasily M. Studitsky

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Short DNA fragments containing single nucleosomes have been extensively employed as simple model experimental systems for analysis of many intranuclear processes, including binding of proteins to nucleosomes, covalent histone modifications, transcription, DNA repair, and ATP-dependent chromatin remodeling. Here we describe several recently developed procedures for obtaining and analysis of mononucleosomes assembled on 200-350-bp DNA fragments.

Original languageEnglish
Title of host publicationChromatin Protocols
Subtitle of host publicationThird Edition
PublisherSpringer New York
Pages15-26
Number of pages12
Volume1288
ISBN (Electronic)9781493924745
ISBN (Print)9781493924738
DOIs
StatePublished - Mar 31 2015

Publication series

NameMethods in molecular biology (Clifton, N.J.)
PublisherHumana Press
ISSN (Print)1064-3745

Keywords

  • Animals
  • Chromatin Assembly and Disassembly
  • Chromatin/genetics
  • DNA/genetics
  • Erythrocytes
  • Histones/metabolism
  • Nucleosomes/chemistry

Fingerprint

Dive into the research topics of 'Preparation and analysis of positioned mononucleosomes'. Together they form a unique fingerprint.

Cite this