Preparation and analysis of positioned mononucleosomes

Olga I. Kulaeva; Vasily M. Studitsky

doi:10.1007/978-1-4939-2474-5_2

Preparation and analysis of positioned mononucleosomes

Olga I. Kulaeva, Vasily M. Studitsky

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Short DNA fragments containing single nucleosomes have been extensively employed as simple model experimental systems for analysis of many intranuclear processes, including binding of proteins to nucleosomes, covalent histone modifications, transcription, DNA repair, and ATP-dependent chromatin remodeling. Here we describe several recently developed procedures for obtaining and analysis of mononucleosomes assembled on 200-350-bp DNA fragments.

Original language	English
Pages (from-to)	15-26
Number of pages	12
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	1288
DOIs	https://doi.org/10.1007/978-1-4939-2474-5_2
State	Published - Mar 31 2015

Keywords

Animals
Chromatin Assembly and Disassembly
Chromatin/genetics
DNA/genetics
Erythrocytes
Histones/metabolism
Nucleosomes/chemistry

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10.1007/978-1-4939-2474-5_2

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abstract = "Short DNA fragments containing single nucleosomes have been extensively employed as simple model experimental systems for analysis of many intranuclear processes, including binding of proteins to nucleosomes, covalent histone modifications, transcription, DNA repair, and ATP-dependent chromatin remodeling. Here we describe several recently developed procedures for obtaining and analysis of mononucleosomes assembled on 200-350-bp DNA fragments.",

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AU - Studitsky, Vasily M.

N1 - Publisher Copyright: © Springer Science+Business Media New York 2015. All rights are reserved.

PY - 2015/3/31

Y1 - 2015/3/31

N2 - Short DNA fragments containing single nucleosomes have been extensively employed as simple model experimental systems for analysis of many intranuclear processes, including binding of proteins to nucleosomes, covalent histone modifications, transcription, DNA repair, and ATP-dependent chromatin remodeling. Here we describe several recently developed procedures for obtaining and analysis of mononucleosomes assembled on 200-350-bp DNA fragments.

AB - Short DNA fragments containing single nucleosomes have been extensively employed as simple model experimental systems for analysis of many intranuclear processes, including binding of proteins to nucleosomes, covalent histone modifications, transcription, DNA repair, and ATP-dependent chromatin remodeling. Here we describe several recently developed procedures for obtaining and analysis of mononucleosomes assembled on 200-350-bp DNA fragments.

KW - Animals

KW - Chromatin Assembly and Disassembly

KW - Chromatin/genetics

KW - DNA/genetics

KW - Erythrocytes

KW - Histones/metabolism

KW - Nucleosomes/chemistry

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U2 - 10.1007/978-1-4939-2474-5_2

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SN - 1064-3745

VL - 1288

SP - 15

EP - 26

JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

ER -

Preparation and analysis of positioned mononucleosomes

Abstract

Keywords

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