Plasma membrane vesicles from bovine adrenal chromaffin cells: Characterization and fusion with chromaffin granules

P. I. Lelkes; D. Naquira; J. E. Friedman; K. Rosenheck; A. S. Schneider

Plasma membrane vesicles from bovine adrenal chromaffin cells: Characterization and fusion with chromaffin granules

P. I. Lelkes, D. Naquira, J. E. Friedman, K. Rosenheck, A. S. Schneider

Cancer Signaling and Microenvironment (CSM)

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Plasma membranes of chromaffin cells are isolated from the bovine adrenal medulla. Treatment of the membranes in the French press gives vesicles of uniform size, ~85% of which are closed. These are oriented right-side-out to ~95%, as indicated by α-Bungarotoxin-binding, enzymatic activities, aggregation by lectins and presence of membrane-associated actin. The vesicles are able to maintain membrane potentials induced by K⁺ and valinomycin. The membrane lipids undergo a phase transition in the 30 ° to 40 °C range. Fusion between these vesicles and intact chromaffin granules is observed by two independent assays. The vesicles are potentially useful for modeling catecholamine release by exocytosis.

Original language	English
Pages (from-to)	143-150
Number of pages	8
Journal	Advances in the Biosciences
Volume	36
Issue number	C
State	Published - 1982

Keywords

Adrenal chromaffin cell
exocytotic fusion
membrane fluidity
membrane potential
membrane sidedness
model system
plasma membrane vesicles

Cite this

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title = "Plasma membrane vesicles from bovine adrenal chromaffin cells: Characterization and fusion with chromaffin granules",

abstract = "Plasma membranes of chromaffin cells are isolated from the bovine adrenal medulla. Treatment of the membranes in the French press gives vesicles of uniform size, ~85% of which are closed. These are oriented right-side-out to ~95%, as indicated by α-Bungarotoxin-binding, enzymatic activities, aggregation by lectins and presence of membrane-associated actin. The vesicles are able to maintain membrane potentials induced by K+ and valinomycin. The membrane lipids undergo a phase transition in the 30 ° to 40 °C range. Fusion between these vesicles and intact chromaffin granules is observed by two independent assays. The vesicles are potentially useful for modeling catecholamine release by exocytosis.",

keywords = "Adrenal chromaffin cell, exocytotic fusion, membrane fluidity, membrane potential, membrane sidedness, model system, plasma membrane vesicles",

author = "Lelkes, {P. I.} and D. Naquira and Friedman, {J. E.} and K. Rosenheck and Schneider, {A. S.}",

year = "1982",

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journal = "Advances in the Biosciences",

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TY - JOUR

T1 - Plasma membrane vesicles from bovine adrenal chromaffin cells

T2 - Characterization and fusion with chromaffin granules

AU - Lelkes, P. I.

AU - Naquira, D.

AU - Friedman, J. E.

AU - Rosenheck, K.

AU - Schneider, A. S.

PY - 1982

Y1 - 1982

N2 - Plasma membranes of chromaffin cells are isolated from the bovine adrenal medulla. Treatment of the membranes in the French press gives vesicles of uniform size, ~85% of which are closed. These are oriented right-side-out to ~95%, as indicated by α-Bungarotoxin-binding, enzymatic activities, aggregation by lectins and presence of membrane-associated actin. The vesicles are able to maintain membrane potentials induced by K+ and valinomycin. The membrane lipids undergo a phase transition in the 30 ° to 40 °C range. Fusion between these vesicles and intact chromaffin granules is observed by two independent assays. The vesicles are potentially useful for modeling catecholamine release by exocytosis.

AB - Plasma membranes of chromaffin cells are isolated from the bovine adrenal medulla. Treatment of the membranes in the French press gives vesicles of uniform size, ~85% of which are closed. These are oriented right-side-out to ~95%, as indicated by α-Bungarotoxin-binding, enzymatic activities, aggregation by lectins and presence of membrane-associated actin. The vesicles are able to maintain membrane potentials induced by K+ and valinomycin. The membrane lipids undergo a phase transition in the 30 ° to 40 °C range. Fusion between these vesicles and intact chromaffin granules is observed by two independent assays. The vesicles are potentially useful for modeling catecholamine release by exocytosis.

KW - Adrenal chromaffin cell

KW - exocytotic fusion

KW - membrane fluidity

KW - membrane potential

KW - membrane sidedness

KW - model system

KW - plasma membrane vesicles

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M3 - Article

AN - SCOPUS:49049131497

SN - 0065-3446

VL - 36

SP - 143

EP - 150

JO - Advances in the Biosciences

JF - Advances in the Biosciences

IS - C

ER -

Plasma membrane vesicles from bovine adrenal chromaffin cells: Characterization and fusion with chromaffin granules

Abstract

Keywords

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