Permissive growth of human adenovirus type 4 vaccine strain-based vector in porcine cell lines

Dong sheng Gao; Xiao jing Li; Wen yan Wan; Hong jie Li; Xiao xue Wang; Xia Yang; Yong tao Li; Hong tao Chang; Lu Chen; Chuan qing Wang; Jun Zhao

doi:10.1016/j.rvsc.2015.12.004

Permissive growth of human adenovirus type 4 vaccine strain-based vector in porcine cell lines

Dong sheng Gao, Xiao jing Li, Wen yan Wan, Hong jie Li, Xiao xue Wang, Xia Yang, Yong tao Li, Hong tao Chang, Lu Chen, Chuan qing Wang, Jun Zhao

Henan Agricultural University

Research output: Contribution to journal › Article › peer-review

Abstract

In recent years, there has been considerable interest in using adenoviruses as live vectors to develop recombinant vaccines. Previous studies have demonstrated the safety and effectiveness of HIV/SIV and influenza vaccine candidates based on human adenovirus type 4 (Ad4) replication-competent vectors in rhesus macaque and human model. To explore the possibility of human Ad4 vaccine strain used as a vector in developing porcine vaccines, the growth properties of replication-competent human Ad4 vaccine strain recombinant encoding EGFP in different porcine cell lines were investigated. All tested cell lines are permissive for Ad4 vaccine strain vector with varied replication efficiency. Thus, human Ad4 based vectors would be promising supplement to adenovirus vectors as a delivery vehicle for recombinant vaccines in swine industry.

Original language	English
Pages (from-to)	83-85
Number of pages	3
Journal	Research in Veterinary Science
Volume	104
DOIs	https://doi.org/10.1016/j.rvsc.2015.12.004
State	Published - Feb 1 2016
Externally published	Yes

Keywords

Adenovirus type 4
Live vector
Porcine cell lines
Recombinant vaccine
Replication

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.rvsc.2015.12.004

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title = "Permissive growth of human adenovirus type 4 vaccine strain-based vector in porcine cell lines",

abstract = "In recent years, there has been considerable interest in using adenoviruses as live vectors to develop recombinant vaccines. Previous studies have demonstrated the safety and effectiveness of HIV/SIV and influenza vaccine candidates based on human adenovirus type 4 (Ad4) replication-competent vectors in rhesus macaque and human model. To explore the possibility of human Ad4 vaccine strain used as a vector in developing porcine vaccines, the growth properties of replication-competent human Ad4 vaccine strain recombinant encoding EGFP in different porcine cell lines were investigated. All tested cell lines are permissive for Ad4 vaccine strain vector with varied replication efficiency. Thus, human Ad4 based vectors would be promising supplement to adenovirus vectors as a delivery vehicle for recombinant vaccines in swine industry.",

keywords = "Adenovirus type 4, Live vector, Porcine cell lines, Recombinant vaccine, Replication",

author = "Gao, {Dong sheng} and Li, {Xiao jing} and Wan, {Wen yan} and Li, {Hong jie} and Wang, {Xiao xue} and Xia Yang and Li, {Yong tao} and Chang, {Hong tao} and Lu Chen and Wang, {Chuan qing} and Jun Zhao",

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doi = "10.1016/j.rvsc.2015.12.004",

language = "English",

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T1 - Permissive growth of human adenovirus type 4 vaccine strain-based vector in porcine cell lines

AU - Gao, Dong sheng

AU - Li, Xiao jing

AU - Wan, Wen yan

AU - Li, Hong jie

AU - Wang, Xiao xue

AU - Yang, Xia

AU - Li, Yong tao

AU - Chang, Hong tao

AU - Chen, Lu

AU - Wang, Chuan qing

AU - Zhao, Jun

PY - 2016/2/1

Y1 - 2016/2/1

N2 - In recent years, there has been considerable interest in using adenoviruses as live vectors to develop recombinant vaccines. Previous studies have demonstrated the safety and effectiveness of HIV/SIV and influenza vaccine candidates based on human adenovirus type 4 (Ad4) replication-competent vectors in rhesus macaque and human model. To explore the possibility of human Ad4 vaccine strain used as a vector in developing porcine vaccines, the growth properties of replication-competent human Ad4 vaccine strain recombinant encoding EGFP in different porcine cell lines were investigated. All tested cell lines are permissive for Ad4 vaccine strain vector with varied replication efficiency. Thus, human Ad4 based vectors would be promising supplement to adenovirus vectors as a delivery vehicle for recombinant vaccines in swine industry.

AB - In recent years, there has been considerable interest in using adenoviruses as live vectors to develop recombinant vaccines. Previous studies have demonstrated the safety and effectiveness of HIV/SIV and influenza vaccine candidates based on human adenovirus type 4 (Ad4) replication-competent vectors in rhesus macaque and human model. To explore the possibility of human Ad4 vaccine strain used as a vector in developing porcine vaccines, the growth properties of replication-competent human Ad4 vaccine strain recombinant encoding EGFP in different porcine cell lines were investigated. All tested cell lines are permissive for Ad4 vaccine strain vector with varied replication efficiency. Thus, human Ad4 based vectors would be promising supplement to adenovirus vectors as a delivery vehicle for recombinant vaccines in swine industry.

KW - Adenovirus type 4

KW - Live vector

KW - Porcine cell lines

KW - Recombinant vaccine

KW - Replication

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DO - 10.1016/j.rvsc.2015.12.004

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AN - SCOPUS:84957624509

SN - 0034-5288

VL - 104

SP - 83

EP - 85

JO - Research in Veterinary Science

JF - Research in Veterinary Science

ER -

Permissive growth of human adenovirus type 4 vaccine strain-based vector in porcine cell lines

Abstract

Keywords

UN SDGs

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