P16 status, pathologic and clinical characteristics, biomolecular signature, and long-term outcomes in head and neck squamous cell carcinomas of unknown primary

Lanea M. Keller; Thomas J. Galloway; Thomas Holdbrook; Karen Ruth; Donghua Yang; Cara Dubyk; Douglas Flieder; Miriam N. Lango; Ranee Mehra; Barbara Burtness; John A. Ridge

doi:10.1002/hed.23514

P16 status, pathologic and clinical characteristics, biomolecular signature, and long-term outcomes in head and neck squamous cell carcinomas of unknown primary

Lanea M. Keller, Thomas J. Galloway, Thomas Holdbrook, Karen Ruth, Donghua Yang, Cara Dubyk, Douglas Flieder, Miriam N. Lango, Ranee Mehra, Barbara Burtness, John A. Ridge

Fox Chase Cancer Center

Research output: Contribution to journal › Article › peer-review

88 Scopus citations

Abstract

Background. The purpose of this study was to report associations between p16 status, clinicopathologic characteristics, and outcomes for head and neck squamous cell carcinoma of unknown primary (CUP).

Methods. Specimens of squamous cell CUP were reanalyzed. Human papillomavirus (HPV) status was determined by p16 stain. A tissue microarray (TMA) was constructed to evaluate biomarkers potentially prognostic in head and neck squamous cell carcinoma (HNSCC).

Results. A majority of the population (n=26; 74%) was p16 positive (1). Prognostic factors benefiting survival were p161 status (p<.0001), absence of macroscopic extracapsular extension (ECE; p=.004), younger age (p=.01), and higher grade (p=0.007). The prognostic implication of worse overall survival (OS) with macroscopic ECE (p = .009) remained significant when limited to patients who were p161 (p=.002). Exploratory TMA between unknown primary and controls suggested a biomolecular difference between squamous cell CUP and known-primary cancer.

Conclusion. The majority of patients with squamous cell CUP were p161, indicative of HPV association. P16 staining and ECE seem to be the most prognostic features in squamous cell CUP.

Original language	English
Pages (from-to)	1677-1684
Number of pages	8
Journal	Head and Neck
Volume	36
Issue number	12
DOIs	https://doi.org/10.1002/hed.23514
State	Published - Dec 1 2014

Keywords

Concurrent chemotherapy
Extracapsular extension (ECE)
Human papillomavirus (HPV)
Tissue microarray
Unknown primary

Access to Document

10.1002/hed.23514

Cite this

Keller, L. M., Galloway, T. J., Holdbrook, T., Ruth, K., Yang, D., Dubyk, C., Flieder, D., Lango, M. N., Mehra, R., Burtness, B., & Ridge, J. A. (2014). P16 status, pathologic and clinical characteristics, biomolecular signature, and long-term outcomes in head and neck squamous cell carcinomas of unknown primary. Head and Neck, 36(12), 1677-1684. https://doi.org/10.1002/hed.23514

@article{fb143c5ebdde40609ee87585d00f9d89,

title = "P16 status, pathologic and clinical characteristics, biomolecular signature, and long-term outcomes in head and neck squamous cell carcinomas of unknown primary",

abstract = "Background. The purpose of this study was to report associations between p16 status, clinicopathologic characteristics, and outcomes for head and neck squamous cell carcinoma of unknown primary (CUP).Methods. Specimens of squamous cell CUP were reanalyzed. Human papillomavirus (HPV) status was determined by p16 stain. A tissue microarray (TMA) was constructed to evaluate biomarkers potentially prognostic in head and neck squamous cell carcinoma (HNSCC).Results. A majority of the population (n=26; 74%) was p16 positive (1). Prognostic factors benefiting survival were p161 status (p<.0001), absence of macroscopic extracapsular extension (ECE; p=.004), younger age (p=.01), and higher grade (p=0.007). The prognostic implication of worse overall survival (OS) with macroscopic ECE (p = .009) remained significant when limited to patients who were p161 (p=.002). Exploratory TMA between unknown primary and controls suggested a biomolecular difference between squamous cell CUP and known-primary cancer.Conclusion. The majority of patients with squamous cell CUP were p161, indicative of HPV association. P16 staining and ECE seem to be the most prognostic features in squamous cell CUP.",

keywords = "Concurrent chemotherapy, Extracapsular extension (ECE), Human papillomavirus (HPV), Tissue microarray, Unknown primary",

author = "Keller, {Lanea M.} and Galloway, {Thomas J.} and Thomas Holdbrook and Karen Ruth and Donghua Yang and Cara Dubyk and Douglas Flieder and Lango, {Miriam N.} and Ranee Mehra and Barbara Burtness and Ridge, {John A.}",

note = "Publisher Copyright: {\textcopyright} 2014 Wiley Periodicals, Inc.",

year = "2014",

month = dec,

day = "1",

doi = "10.1002/hed.23514",

language = "English",

volume = "36",

pages = "1677--1684",

journal = "Head and Neck",

issn = "1043-3074",

publisher = "John Wiley & Sons Inc.",

number = "12",

}

Keller, LM, Galloway, TJ, Holdbrook, T, Ruth, K, Yang, D, Dubyk, C, Flieder, D, Lango, MN, Mehra, R, Burtness, B & Ridge, JA 2014, 'P16 status, pathologic and clinical characteristics, biomolecular signature, and long-term outcomes in head and neck squamous cell carcinomas of unknown primary', Head and Neck, vol. 36, no. 12, pp. 1677-1684. https://doi.org/10.1002/hed.23514

TY - JOUR

T1 - P16 status, pathologic and clinical characteristics, biomolecular signature, and long-term outcomes in head and neck squamous cell carcinomas of unknown primary

AU - Keller, Lanea M.

AU - Galloway, Thomas J.

AU - Holdbrook, Thomas

AU - Ruth, Karen

AU - Yang, Donghua

AU - Dubyk, Cara

AU - Flieder, Douglas

AU - Lango, Miriam N.

AU - Mehra, Ranee

AU - Burtness, Barbara

AU - Ridge, John A.

PY - 2014/12/1

Y1 - 2014/12/1

N2 - Background. The purpose of this study was to report associations between p16 status, clinicopathologic characteristics, and outcomes for head and neck squamous cell carcinoma of unknown primary (CUP).Methods. Specimens of squamous cell CUP were reanalyzed. Human papillomavirus (HPV) status was determined by p16 stain. A tissue microarray (TMA) was constructed to evaluate biomarkers potentially prognostic in head and neck squamous cell carcinoma (HNSCC).Results. A majority of the population (n=26; 74%) was p16 positive (1). Prognostic factors benefiting survival were p161 status (p<.0001), absence of macroscopic extracapsular extension (ECE; p=.004), younger age (p=.01), and higher grade (p=0.007). The prognostic implication of worse overall survival (OS) with macroscopic ECE (p = .009) remained significant when limited to patients who were p161 (p=.002). Exploratory TMA between unknown primary and controls suggested a biomolecular difference between squamous cell CUP and known-primary cancer.Conclusion. The majority of patients with squamous cell CUP were p161, indicative of HPV association. P16 staining and ECE seem to be the most prognostic features in squamous cell CUP.

AB - Background. The purpose of this study was to report associations between p16 status, clinicopathologic characteristics, and outcomes for head and neck squamous cell carcinoma of unknown primary (CUP).Methods. Specimens of squamous cell CUP were reanalyzed. Human papillomavirus (HPV) status was determined by p16 stain. A tissue microarray (TMA) was constructed to evaluate biomarkers potentially prognostic in head and neck squamous cell carcinoma (HNSCC).Results. A majority of the population (n=26; 74%) was p16 positive (1). Prognostic factors benefiting survival were p161 status (p<.0001), absence of macroscopic extracapsular extension (ECE; p=.004), younger age (p=.01), and higher grade (p=0.007). The prognostic implication of worse overall survival (OS) with macroscopic ECE (p = .009) remained significant when limited to patients who were p161 (p=.002). Exploratory TMA between unknown primary and controls suggested a biomolecular difference between squamous cell CUP and known-primary cancer.Conclusion. The majority of patients with squamous cell CUP were p161, indicative of HPV association. P16 staining and ECE seem to be the most prognostic features in squamous cell CUP.

KW - Concurrent chemotherapy

KW - Extracapsular extension (ECE)

KW - Human papillomavirus (HPV)

KW - Tissue microarray

KW - Unknown primary

UR - http://www.scopus.com/inward/record.url?scp=84912009604&partnerID=8YFLogxK

UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=purepublist2023&SrcAuth=WosAPI&KeyUT=WOS:000344801200005&DestLinkType=FullRecord&DestApp=WOS

U2 - 10.1002/hed.23514

DO - 10.1002/hed.23514

M3 - Article

C2 - 24115269

SN - 1043-3074

VL - 36

SP - 1677

EP - 1684

JO - Head and Neck

JF - Head and Neck

IS - 12

ER -

P16 status, pathologic and clinical characteristics, biomolecular signature, and long-term outcomes in head and neck squamous cell carcinomas of unknown primary

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this