TY - JOUR
T1 - Nucleosome remodeling induced by RNA polymerase II
T2 - Loss of the H2A/H2B dimer during transcription
AU - Kireeva, Maria L.
AU - Walter, Wendy
AU - Tchernajenko, Vladimir
AU - Bondarenko, Vladimir
AU - Kashlev, Mikhail
AU - Studitsky, Vasily M.
PY - 2002
Y1 - 2002
N2 - RNA polymerase II (Pol II) must transcribe genes in a chromatin environment in vivo. We examined transcription by Pol II through nucleosome cores in vitro. At physiological and lower ionic strengths, a mononucleosome imposes a strong block to elongation, which is relieved at increased ionic strength. Passage of Pol II causes a quantitative loss of one H2A/H2B dimer but does not alter the location of the nucleosome. In contrast, bacteriophage SP6 RNA polymerase (RNAP) efficiently transcribes through the same nucleosome under physiological conditions, and the histone octamer is transferred behind SP6 RNAP. Thus, the mechanisms for transcription through the nucleosome by Pol II and SP6 RNAP are clearly different. Moreover, Pol II leaves behind an imprint of disrupted chromatin structure.
AB - RNA polymerase II (Pol II) must transcribe genes in a chromatin environment in vivo. We examined transcription by Pol II through nucleosome cores in vitro. At physiological and lower ionic strengths, a mononucleosome imposes a strong block to elongation, which is relieved at increased ionic strength. Passage of Pol II causes a quantitative loss of one H2A/H2B dimer but does not alter the location of the nucleosome. In contrast, bacteriophage SP6 RNA polymerase (RNAP) efficiently transcribes through the same nucleosome under physiological conditions, and the histone octamer is transferred behind SP6 RNAP. Thus, the mechanisms for transcription through the nucleosome by Pol II and SP6 RNAP are clearly different. Moreover, Pol II leaves behind an imprint of disrupted chromatin structure.
KW - DNA-Directed RNA Polymerases/metabolism
KW - Dimerization
KW - Histones/metabolism
KW - Macromolecular Substances
KW - Models, Genetic
KW - Nucleosomes/metabolism
KW - Oligonucleotides/genetics
KW - RNA Polymerase II/metabolism
KW - Transcription, Genetic/physiology
UR - http://www.scopus.com/inward/record.url?scp=0036203807&partnerID=8YFLogxK
U2 - 10.1016/S1097-2765(02)00472-0
DO - 10.1016/S1097-2765(02)00472-0
M3 - Article
C2 - 11931762
AN - SCOPUS:0036203807
SN - 1097-2765
VL - 9
SP - 541
EP - 552
JO - Molecular Cell
JF - Molecular Cell
IS - 3
ER -