TY - JOUR
T1 - Nucleolin is a histone chaperone with FACT-like activity and assists remodeling of nucleosomes
AU - Angelov, Dimitar
AU - Bondarenko, Vladimir A.
AU - Almagro, Sébastien
AU - Menoni, Hervé
AU - Mongélard, Fabien
AU - Hans, Fabienne
AU - Mietton, Flore
AU - Studitsky, Vasily M.
AU - Hamiche, Ali
AU - Dimitrov, Stefan
AU - Bouvet, Philippe
PY - 2006/4/19
Y1 - 2006/4/19
N2 - Remodeling machines play an essential role in the control of gene expression, but how their activity is regulated is not known. Here we report that the nuclear protein nucleolin possesses a histone chaperone activity and that this factor greatly enhances the activity of the chromatin remodeling machineries SWI/SNF and ACF. Interestingly, nucleolin is able to induce the remodeling by SWI/SNF of macroH2A, but not of H2ABbd nucleosomes, which are otherwise resistant to remodeling. This new histone chaperone promotes the destabilization of the histone octamer, helping the dissociation of a H2A-H2B dimer, and stimulates the SWI/SNF-mediated transfer of H2A-H2B dimers. Furthermore, nucleolin facilitates transcription through the nucleosome, which is reminiscent of the activity of the FACT complex. This work defines new functions for histone chaperones in chromatin remodeling and regulation of transcription and explains how nucleolin could act on transcription.
AB - Remodeling machines play an essential role in the control of gene expression, but how their activity is regulated is not known. Here we report that the nuclear protein nucleolin possesses a histone chaperone activity and that this factor greatly enhances the activity of the chromatin remodeling machineries SWI/SNF and ACF. Interestingly, nucleolin is able to induce the remodeling by SWI/SNF of macroH2A, but not of H2ABbd nucleosomes, which are otherwise resistant to remodeling. This new histone chaperone promotes the destabilization of the histone octamer, helping the dissociation of a H2A-H2B dimer, and stimulates the SWI/SNF-mediated transfer of H2A-H2B dimers. Furthermore, nucleolin facilitates transcription through the nucleosome, which is reminiscent of the activity of the FACT complex. This work defines new functions for histone chaperones in chromatin remodeling and regulation of transcription and explains how nucleolin could act on transcription.
KW - Nucleolin
KW - Nucleosome
KW - Remodeling
KW - Transcription
KW - macroH2A and H2ABbd histone variants
UR - http://www.scopus.com/inward/record.url?scp=33646164896&partnerID=8YFLogxK
UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=purepublist2023&SrcAuth=WosAPI&KeyUT=WOS:000237589800009&DestLinkType=FullRecord&DestApp=WOS
U2 - 10.1038/sj.emboj.7601046
DO - 10.1038/sj.emboj.7601046
M3 - Article
C2 - 16601700
SN - 0261-4189
VL - 25
SP - 1669
EP - 1679
JO - EMBO Journal
JF - EMBO Journal
IS - 8
ER -