Novel, Rapid, and Inexpensive Cell-Based Quantification of Antimalarial Drug Efficacy

Tyler N. Bennett; Michelle Paguio; Bojana Gligorijevic; Clement Seudieu; Andrew D. Kosar; Eugene Davidson; Paul D. Roepe

doi:10.1128/AAC.48.5.1807-1810.2004

Novel, Rapid, and Inexpensive Cell-Based Quantification of Antimalarial Drug Efficacy

Tyler N. Bennett
, Michelle Paguio
, Bojana Gligorijevic
, Clement Seudieu
, Andrew D. Kosar
, Eugene Davidson
, Paul D. Roepe

Cancer Signaling and Microenvironment (CSM)

Georgetown University

Research output: Contribution to journal › Article › peer-review

240 Scopus citations

Abstract

We report on the development of a new SYBR Green I-based plate assay for analyzing the activities of antimalarial drugs against intraerythrocytic Plasmodium falciparum. This assay is considerably faster, less labor-intensive, and less expensive than conventional radiotracer (e.g., [ ³H]hypoxanthine and [³H]ethanolamine)-based assays or P. falciparum lactate dehydrogenase activity-based assays. The assay significantly improves the pace at which antimalarial drug discovery efforts may proceed.

Original language	English
Pages (from-to)	1807-1810
Number of pages	4
Journal	Antimicrobial Agents and Chemotherapy
Volume	48
Issue number	5
DOIs	https://doi.org/10.1128/AAC.48.5.1807-1810.2004
State	Published - May 2004

Keywords

Animals
Antimalarials/economics
Benzothiazoles
Chloroquine/pharmacology
Diamines
Drug Evaluation, Preclinical/economics
Drug Resistance
Erythrocytes/parasitology
Fluorescent Dyes
Humans
Microscopy, Confocal
Organic Chemicals
Plasmodium falciparum/drug effects
Quinolines

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10.1128/AAC.48.5.1807-1810.2004

Cite this

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title = "Novel, Rapid, and Inexpensive Cell-Based Quantification of Antimalarial Drug Efficacy",

abstract = "We report on the development of a new SYBR Green I-based plate assay for analyzing the activities of antimalarial drugs against intraerythrocytic Plasmodium falciparum. This assay is considerably faster, less labor-intensive, and less expensive than conventional radiotracer (e.g., [ 3H]hypoxanthine and [3H]ethanolamine)-based assays or P. falciparum lactate dehydrogenase activity-based assays. The assay significantly improves the pace at which antimalarial drug discovery efforts may proceed.",

keywords = "Animals, Antimalarials/economics, Benzothiazoles, Chloroquine/pharmacology, Diamines, Drug Evaluation, Preclinical/economics, Drug Resistance, Erythrocytes/parasitology, Fluorescent Dyes, Humans, Microscopy, Confocal, Organic Chemicals, Plasmodium falciparum/drug effects, Quinolines",

author = "Bennett, \{Tyler N.\} and Michelle Paguio and Bojana Gligorijevic and Clement Seudieu and Kosar, \{Andrew D.\} and Eugene Davidson and Roepe, \{Paul D.\}",

year = "2004",

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language = "English",

volume = "48",

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journal = "Antimicrobial Agents and Chemotherapy",

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publisher = "American Society for Microbiology",

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TY - JOUR

T1 - Novel, Rapid, and Inexpensive Cell-Based Quantification of Antimalarial Drug Efficacy

AU - Bennett, Tyler N.

AU - Paguio, Michelle

AU - Gligorijevic, Bojana

AU - Seudieu, Clement

AU - Kosar, Andrew D.

AU - Davidson, Eugene

AU - Roepe, Paul D.

PY - 2004/5

Y1 - 2004/5

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AB - We report on the development of a new SYBR Green I-based plate assay for analyzing the activities of antimalarial drugs against intraerythrocytic Plasmodium falciparum. This assay is considerably faster, less labor-intensive, and less expensive than conventional radiotracer (e.g., [ 3H]hypoxanthine and [3H]ethanolamine)-based assays or P. falciparum lactate dehydrogenase activity-based assays. The assay significantly improves the pace at which antimalarial drug discovery efforts may proceed.

KW - Animals

KW - Antimalarials/economics

KW - Benzothiazoles

KW - Chloroquine/pharmacology

KW - Diamines

KW - Drug Evaluation, Preclinical/economics

KW - Drug Resistance

KW - Erythrocytes/parasitology

KW - Fluorescent Dyes

KW - Humans

KW - Microscopy, Confocal

KW - Organic Chemicals

KW - Plasmodium falciparum/drug effects

KW - Quinolines

UR - https://www.scopus.com/pages/publications/2142662134

U2 - 10.1128/AAC.48.5.1807-1810.2004

DO - 10.1128/AAC.48.5.1807-1810.2004

M3 - Article

C2 - 15105139

SN - 0066-4804

VL - 48

SP - 1807

EP - 1810

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

IS - 5

ER -

Novel, Rapid, and Inexpensive Cell-Based Quantification of Antimalarial Drug Efficacy

Abstract

Keywords

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