Abstract
We report on the development of a new SYBR Green I-based plate assay for analyzing the activities of antimalarial drugs against intraerythrocytic Plasmodium falciparum. This assay is considerably faster, less labor-intensive, and less expensive than conventional radiotracer (e.g., [ 3H]hypoxanthine and [3H]ethanolamine)-based assays or P. falciparum lactate dehydrogenase activity-based assays. The assay significantly improves the pace at which antimalarial drug discovery efforts may proceed.
Original language | English |
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Pages (from-to) | 1807-1810 |
Number of pages | 4 |
Journal | Antimicrobial Agents and Chemotherapy |
Volume | 48 |
Issue number | 5 |
DOIs | |
State | Published - May 2004 |
Keywords
- Animals
- Antimalarials/economics
- Benzothiazoles
- Chloroquine/pharmacology
- Diamines
- Drug Evaluation, Preclinical/economics
- Drug Resistance
- Erythrocytes/parasitology
- Fluorescent Dyes
- Humans
- Microscopy, Confocal
- Organic Chemicals
- Plasmodium falciparum/drug effects
- Quinolines