Novel, Rapid, and Inexpensive Cell-Based Quantification of Antimalarial Drug Efficacy

Tyler N. Bennett, Michelle Paguio, Bojana Gligorijevic, Clement Seudieu, Andrew D. Kosar, Eugene Davidson, Paul D. Roepe

Research output: Contribution to journalArticlepeer-review

232 Scopus citations

Abstract

We report on the development of a new SYBR Green I-based plate assay for analyzing the activities of antimalarial drugs against intraerythrocytic Plasmodium falciparum. This assay is considerably faster, less labor-intensive, and less expensive than conventional radiotracer (e.g., [ 3H]hypoxanthine and [3H]ethanolamine)-based assays or P. falciparum lactate dehydrogenase activity-based assays. The assay significantly improves the pace at which antimalarial drug discovery efforts may proceed.

Original languageEnglish
Pages (from-to)1807-1810
Number of pages4
JournalAntimicrobial Agents and Chemotherapy
Volume48
Issue number5
DOIs
StatePublished - May 2004

Keywords

  • Animals
  • Antimalarials/economics
  • Benzothiazoles
  • Chloroquine/pharmacology
  • Diamines
  • Drug Evaluation, Preclinical/economics
  • Drug Resistance
  • Erythrocytes/parasitology
  • Fluorescent Dyes
  • Humans
  • Microscopy, Confocal
  • Organic Chemicals
  • Plasmodium falciparum/drug effects
  • Quinolines

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