Novel functional activities of anti-DNA autoantibodies from sera of patients with lymphoproliferative and autoimmune diseases

A. V. Kozyr'; A. V. Kolesnikov; E. S. Aleksandrova; L. P. Sashchenko; N. V. Gnuchev; P. V. Favorov; M. A. Kotelnikov; E. I. Iakhnina; Igor Astsaturov; T. B. Prokaeva; Z. S. Alekberova; S. V. Suchkov; A. G. Gabibov

doi:10.1007/BF02787708

Novel functional activities of anti-DNA autoantibodies from sera of patients with lymphoproliferative and autoimmune diseases

A. V. Kozyr', A. V. Kolesnikov, E. S. Aleksandrova, L. P. Sashchenko, N. V. Gnuchev, P. V. Favorov, M. A. Kotelnikov, E. I. Iakhnina, Igor Astsaturov, T. B. Prokaeva, Z. S. Alekberova, S. V. Suchkov, A. G. Gabibov

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103 Scopus citations

Abstract

DNA-hydrolyzing activity of IgG autoantibodies from sera of patients with various types of lymphoproliferative diseases was investigated. The association of DNA-hydrolyzing activity with the antibody (Ab) fraction has been proved by newly developed affinity-capture assay. Study of abzyme incidence in blood tumors and systemic lupus erythematosis (SLE) revealed linkage of anti-DNA Ab catalysts to mature B-cell tumors, and increased probability of DNA-abzymes formation on the background of autoimmune manifestations. These data suggest possible similarity between mechanisms of abzyme formation in SLE and B-cell lymphomas. A new mechanism of formation of DNA-specific catalytic Abs has been proposed based on the increased crossreactivity of polyclonal DNA-abzymes to DNA-depleted nuclear matrix proteins. The possibility of the abzyme production as Ab to the energetically destabilized ground state of the antigen has been discussed. Preliminary results were obtained that indicate the complement-independent cytotoxicity of anti-DNA autoantibodies isolated from blood of patients with SLE and chronic lymphocytic leukemia.

Original language	English
Pages (from-to)	45-61
Number of pages	17
Journal	Applied Biochemistry and Biotechnology
Volume	75
Issue number	1
DOIs	https://doi.org/10.1007/BF02787708
State	Published - 1998

Keywords

Affinity-capture assay
Autoantibody cytotoxicity
Chronic lymphocytic leukemia
DNA- protein complexes
DNA-hydrolyzing antibodies
Lymphoproliferative diseases
Nuclear matrix

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10.1007/BF02787708

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Kozyr', A. V., Kolesnikov, A. V., Aleksandrova, E. S., Sashchenko, L. P., Gnuchev, N. V., Favorov, P. V., Kotelnikov, M. A., Iakhnina, E. I., Astsaturov, I., Prokaeva, T. B., Alekberova, Z. S., Suchkov, S. V., & Gabibov, A. G. (1998). Novel functional activities of anti-DNA autoantibodies from sera of patients with lymphoproliferative and autoimmune diseases. Applied Biochemistry and Biotechnology, 75(1), 45-61. https://doi.org/10.1007/BF02787708

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title = "Novel functional activities of anti-DNA autoantibodies from sera of patients with lymphoproliferative and autoimmune diseases",

abstract = "DNA-hydrolyzing activity of IgG autoantibodies from sera of patients with various types of lymphoproliferative diseases was investigated. The association of DNA-hydrolyzing activity with the antibody (Ab) fraction has been proved by newly developed affinity-capture assay. Study of abzyme incidence in blood tumors and systemic lupus erythematosis (SLE) revealed linkage of anti-DNA Ab catalysts to mature B-cell tumors, and increased probability of DNA-abzymes formation on the background of autoimmune manifestations. These data suggest possible similarity between mechanisms of abzyme formation in SLE and B-cell lymphomas. A new mechanism of formation of DNA-specific catalytic Abs has been proposed based on the increased crossreactivity of polyclonal DNA-abzymes to DNA-depleted nuclear matrix proteins. The possibility of the abzyme production as Ab to the energetically destabilized ground state of the antigen has been discussed. Preliminary results were obtained that indicate the complement-independent cytotoxicity of anti-DNA autoantibodies isolated from blood of patients with SLE and chronic lymphocytic leukemia.",

keywords = "Affinity-capture assay, Autoantibody cytotoxicity, Chronic lymphocytic leukemia, DNA- protein complexes, DNA-hydrolyzing antibodies, Lymphoproliferative diseases, Nuclear matrix",

author = "Kozyr', {A. V.} and Kolesnikov, {A. V.} and Aleksandrova, {E. S.} and Sashchenko, {L. P.} and Gnuchev, {N. V.} and Favorov, {P. V.} and Kotelnikov, {M. A.} and Iakhnina, {E. I.} and Igor Astsaturov and Prokaeva, {T. B.} and Alekberova, {Z. S.} and Suchkov, {S. V.} and Gabibov, {A. G.}",

year = "1998",

doi = "10.1007/BF02787708",

language = "English",

volume = "75",

pages = "45--61",

journal = "Applied Biochemistry and Biotechnology",

issn = "0273-2289",

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number = "1",

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Kozyr', AV, Kolesnikov, AV, Aleksandrova, ES, Sashchenko, LP, Gnuchev, NV, Favorov, PV, Kotelnikov, MA, Iakhnina, EI, Astsaturov, I, Prokaeva, TB, Alekberova, ZS, Suchkov, SV & Gabibov, AG 1998, 'Novel functional activities of anti-DNA autoantibodies from sera of patients with lymphoproliferative and autoimmune diseases', Applied Biochemistry and Biotechnology, vol. 75, no. 1, pp. 45-61. https://doi.org/10.1007/BF02787708

TY - JOUR

T1 - Novel functional activities of anti-DNA autoantibodies from sera of patients with lymphoproliferative and autoimmune diseases

AU - Kozyr', A. V.

AU - Kolesnikov, A. V.

AU - Aleksandrova, E. S.

AU - Sashchenko, L. P.

AU - Gnuchev, N. V.

AU - Favorov, P. V.

AU - Kotelnikov, M. A.

AU - Iakhnina, E. I.

AU - Astsaturov, Igor

AU - Prokaeva, T. B.

AU - Alekberova, Z. S.

AU - Suchkov, S. V.

AU - Gabibov, A. G.

PY - 1998

Y1 - 1998

N2 - DNA-hydrolyzing activity of IgG autoantibodies from sera of patients with various types of lymphoproliferative diseases was investigated. The association of DNA-hydrolyzing activity with the antibody (Ab) fraction has been proved by newly developed affinity-capture assay. Study of abzyme incidence in blood tumors and systemic lupus erythematosis (SLE) revealed linkage of anti-DNA Ab catalysts to mature B-cell tumors, and increased probability of DNA-abzymes formation on the background of autoimmune manifestations. These data suggest possible similarity between mechanisms of abzyme formation in SLE and B-cell lymphomas. A new mechanism of formation of DNA-specific catalytic Abs has been proposed based on the increased crossreactivity of polyclonal DNA-abzymes to DNA-depleted nuclear matrix proteins. The possibility of the abzyme production as Ab to the energetically destabilized ground state of the antigen has been discussed. Preliminary results were obtained that indicate the complement-independent cytotoxicity of anti-DNA autoantibodies isolated from blood of patients with SLE and chronic lymphocytic leukemia.

AB - DNA-hydrolyzing activity of IgG autoantibodies from sera of patients with various types of lymphoproliferative diseases was investigated. The association of DNA-hydrolyzing activity with the antibody (Ab) fraction has been proved by newly developed affinity-capture assay. Study of abzyme incidence in blood tumors and systemic lupus erythematosis (SLE) revealed linkage of anti-DNA Ab catalysts to mature B-cell tumors, and increased probability of DNA-abzymes formation on the background of autoimmune manifestations. These data suggest possible similarity between mechanisms of abzyme formation in SLE and B-cell lymphomas. A new mechanism of formation of DNA-specific catalytic Abs has been proposed based on the increased crossreactivity of polyclonal DNA-abzymes to DNA-depleted nuclear matrix proteins. The possibility of the abzyme production as Ab to the energetically destabilized ground state of the antigen has been discussed. Preliminary results were obtained that indicate the complement-independent cytotoxicity of anti-DNA autoantibodies isolated from blood of patients with SLE and chronic lymphocytic leukemia.

KW - Affinity-capture assay

KW - Autoantibody cytotoxicity

KW - Chronic lymphocytic leukemia

KW - DNA- protein complexes

KW - DNA-hydrolyzing antibodies

KW - Lymphoproliferative diseases

KW - Nuclear matrix

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U2 - 10.1007/BF02787708

DO - 10.1007/BF02787708

M3 - Article

C2 - 10214696

SN - 0273-2289

VL - 75

SP - 45

EP - 61

JO - Applied Biochemistry and Biotechnology

JF - Applied Biochemistry and Biotechnology

IS - 1

ER -

Novel functional activities of anti-DNA autoantibodies from sera of patients with lymphoproliferative and autoimmune diseases

Abstract

Keywords

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