TY - JOUR
T1 - Non-specific modulation of the immune response with liposomal meningococcal lipopolysaccharide
T2 - role of different cells and cytokines
AU - Petrov, Alexander B.
AU - Kolenko, Vladimir M.
AU - Koshkina, Nadezhda V.
AU - Zakirov, Muxim M.
AU - Bugaev, Lev V.
AU - Semenova, Irina B.
AU - Wiertz, Emmanuel J.H.J.
AU - Poolman, Jan T.
PY - 1994
Y1 - 1994
N2 - The immunomodulating action of Neisseria meningitidis lipopolysaccharide (LPS) incorporated into liposomes and the activation of different populations of immunocompetent cells or the secretion of cytokines were studied. LPS stimulated an anti-sheep red blood cell (SRBC) plaque-forming cell response in the spleen of mice after simultaneous injection of LPS and SRBC but if LPS was administered 3 days before the immunization with SRBC the response to SRBC was strongly suppressed. After the incorporation of LPS into liposomes the stimulation index was increased from 6 to 19 and the liposomal LPS did not suppress the immune response to SRBC. The incorporation of LPS into liposomes leads to enhancement of B-mitogenic properties of LPS, as liposomal LPS stimulated the proliferation of splenocytes in mice better than free LPS and has no influence on the thymocytes. The liposomal LPS induced more prolonged and significant accumulation of IgM-secreting cells in the spleen of mice in comparison with the free LPS. Liposomal LPS also induced more active accumulation of IFN-γ in human peripheral blood mononuclear cells and less active accumulation of monokines, contributing to the realization of the toxic properties of endotoxin (IL-1α, TNF-α, IL-6 and GM-CSF). These results demonstrated that the incorporation of N. meningitidis LPS into liposomes dramatically changed its immunomodulating activity. The data obtained are important for the construction of an adjuvant formulation for synthetic immunogens capable of inducing genetically unrestricted immune responses.
AB - The immunomodulating action of Neisseria meningitidis lipopolysaccharide (LPS) incorporated into liposomes and the activation of different populations of immunocompetent cells or the secretion of cytokines were studied. LPS stimulated an anti-sheep red blood cell (SRBC) plaque-forming cell response in the spleen of mice after simultaneous injection of LPS and SRBC but if LPS was administered 3 days before the immunization with SRBC the response to SRBC was strongly suppressed. After the incorporation of LPS into liposomes the stimulation index was increased from 6 to 19 and the liposomal LPS did not suppress the immune response to SRBC. The incorporation of LPS into liposomes leads to enhancement of B-mitogenic properties of LPS, as liposomal LPS stimulated the proliferation of splenocytes in mice better than free LPS and has no influence on the thymocytes. The liposomal LPS induced more prolonged and significant accumulation of IgM-secreting cells in the spleen of mice in comparison with the free LPS. Liposomal LPS also induced more active accumulation of IFN-γ in human peripheral blood mononuclear cells and less active accumulation of monokines, contributing to the realization of the toxic properties of endotoxin (IL-1α, TNF-α, IL-6 and GM-CSF). These results demonstrated that the incorporation of N. meningitidis LPS into liposomes dramatically changed its immunomodulating activity. The data obtained are important for the construction of an adjuvant formulation for synthetic immunogens capable of inducing genetically unrestricted immune responses.
KW - Adjuvants, Immunologic
KW - Animals
KW - B-Lymphocytes/immunology
KW - Bone Marrow Cells
KW - Bone Marrow/immunology
KW - Cells, Cultured
KW - Cytokines/biosynthesis
KW - Drug Carriers
KW - Erythrocytes/immunology
KW - Hemolytic Plaque Technique
KW - Humans
KW - Immunosuppression Therapy
KW - Leukocytes, Mononuclear/immunology
KW - Lipopolysaccharides/administration & dosage
KW - Liposomes/immunology
KW - Lymphocyte Activation
KW - Mice
KW - Mice, Inbred CBA
KW - Neisseria meningitidis/immunology
KW - Pilot Projects
KW - Spleen/cytology
KW - Thymus Gland/cytology
UR - http://www.scopus.com/inward/record.url?scp=0027937071&partnerID=8YFLogxK
UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=purepublist2023&SrcAuth=WosAPI&KeyUT=WOS:A1994PC67300002&DestLinkType=FullRecord&DestApp=WOS
U2 - 10.1016/0264-410X(94)90174-0
DO - 10.1016/0264-410X(94)90174-0
M3 - Article
C2 - 7998414
SN - 0264-410X
VL - 12
SP - 1064
EP - 1070
JO - Vaccine
JF - Vaccine
IS - 12
ER -