@inbook{cd7b69b5014340a0b3bb80d6a90ff716,
title = "Monocyte Adhesion Assays for Detecting Endothelial Cell Activation in Vascular Inflammation and Atherosclerosis",
abstract = "Monocyte adhesion assay, a fluorescence-based method, enables the detection and quantification of monocyte adhesion to endothelial cell (EC) monolayers in vitro and measures EC activation. We describe in this chapter a monocyte adhesion assay based on two published papers from our laboratory that can be effectively used in studying the mechanisms of both pro- and anti-inflammatory cytokines in EC activation. Endothelial cell monolayers are cultured and treated with desired drug, cytokines, or other stimuli and incubated with fluorescently labeled monocytes.",
keywords = "Atherosclerosis/metabolism, Cell Adhesion, Cells, Cultured, Endothelial Cells, Endothelium, Vascular/metabolism, Humans, Inflammation/metabolism, Monocytes/metabolism",
author = "Keman Xu and Fatma Saaoud and Stephanie Yu and Charles Drummer and Ying Shao and Yu Sun and Yifan Lu and Jianxin Sun and Jun Yu and Xiaohua Jiang and Hong Wang and Xiaofeng Yang",
note = "{\textcopyright} 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-1924-7_10",
language = "English",
volume = "2419",
series = "Methods in molecular biology (Clifton, N.J.)",
publisher = "Humana Press Inc.",
pages = "169--182",
booktitle = "Methods in Molecular Biology",
address = "United States",
}