MMP-13 In-Vivo molecular imaging reveals early expression in lung adenocarcinoma

Mathieu Salaün, Jing Peng, Harvey H. Hensley, Navid Roder, Douglas B. Flieder, Solène Houlle-Crépin, Olivia Abramovici-Roels, Jean Christophe Sabourin, Luc Thiberville, Margie L. Clapper

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Introduction Several matrix metalloproteinases (MMPs) are overexpressed in lung cancer and may serve as potential targets for the development of bioactivable probes for molecular imaging. Objective To characterize and monitor the activity of MMPs during the progression of lung adenocarcinoma. Methods K-rasLSL-G12D mice were imaged serially during the development of adenocarcinomas using fluorescence molecular tomography (FMT) and a probe specific for MMP-2, -3, -9 and -13. Lung tumors were identified using FMT and MRI co-registration, and the probe concentration in each tumor was assessed at each time-point. The expression of Mmp2, -3, -9, -13 was quantified by qRT-PCR using RNA isolated from microdissected tumor cells. Immunohistochemical staining of overexpressed MMPs in animals was assessed on human lung tumors. Results In mice, 7 adenomas and 5 adenocarcinomas showed an increase in fluorescent signal on successive FMT scans, starting between weeks 4 and 8. qRT-PCR assays revealed significant overexpression of only Mmp-13 in mice lung tumors. In human tumors, a high MMP-13 immunostaining index was found in tumor cells from invasive lesions (24/27), but in none of the non-invasive (0/4) (p=0.001). Conclusion MMP-13 is detected in early pulmonary invasive adenocarcinomas and may be a potential target for molecular imaging of lung cancer.

Original languageEnglish
Article numbere0132960
Pages (from-to)e0132960
JournalPLoS ONE
Volume10
Issue number7
DOIs
StatePublished - Jul 20 2015

Keywords

  • Adenocarcinoma/metabolism
  • Adenoma/metabolism
  • Animals
  • Humans
  • Immunohistochemistry
  • Lung Neoplasms/metabolism
  • Lung/metabolism
  • Magnetic Resonance Imaging
  • Matrix Metalloproteinase 13/genetics
  • Matrix Metalloproteinases/genetics
  • Mice
  • Optical Imaging
  • RNA, Neoplasm/analysis
  • Real-Time Polymerase Chain Reaction
  • Tomography, X-Ray Computed
  • ras Proteins/genetics

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