TY - JOUR
T1 - Mechanism of chromatin remodeling and recovery during passage of RNA polymerase II
AU - Kulaeva, Olga I.
AU - Gaykalova, Daria A.
AU - Pestov, Nikolai A.
AU - Golovastov, Viktor V.
AU - Vassylyev, Dmitry G.
AU - Artsimovitch, Irina
AU - Studitsky, Vasily M.
PY - 2009/12
Y1 - 2009/12
N2 - Transcription of eukaryotic genes by RNA polymerase II (Pol II) is typically accompanied by nucleosome survival and minimal exchange of histones H3 and H4. The mechanism of nucleosome survival and recovery of chromatin structure remains obscure. Here we show how transcription through chromatin by Pol II is uniquely coupled with nucleosome survival. Structural modeling and functional analysis of the intermediates of transcription through a nucleosome indicated that when Pol II approaches an area of strong DNA-histone interactions, a small intranucleosomal DNA loop (zero-size or-loop) containing transcribing enzyme is formed. During formation of the-loop, the recovery of DNA-histone interactions behind Pol II is tightly coupled with their disruption ahead of the enzyme. This coupling is a distinct feature of the Pol II-type mechanism that allows further transcription through the nucleosome, prevents nucleosome translocation and minimizes displacement of H3 and H4 histones from DNA during enzyme passage.
AB - Transcription of eukaryotic genes by RNA polymerase II (Pol II) is typically accompanied by nucleosome survival and minimal exchange of histones H3 and H4. The mechanism of nucleosome survival and recovery of chromatin structure remains obscure. Here we show how transcription through chromatin by Pol II is uniquely coupled with nucleosome survival. Structural modeling and functional analysis of the intermediates of transcription through a nucleosome indicated that when Pol II approaches an area of strong DNA-histone interactions, a small intranucleosomal DNA loop (zero-size or-loop) containing transcribing enzyme is formed. During formation of the-loop, the recovery of DNA-histone interactions behind Pol II is tightly coupled with their disruption ahead of the enzyme. This coupling is a distinct feature of the Pol II-type mechanism that allows further transcription through the nucleosome, prevents nucleosome translocation and minimizes displacement of H3 and H4 histones from DNA during enzyme passage.
KW - Chromatin Assembly and Disassembly
KW - DNA/metabolism
KW - Escherichia coli Proteins/metabolism
KW - Escherichia coli/physiology
KW - Histones/metabolism
KW - Nucleosomes/metabolism
KW - RNA Polymerase II/metabolism
KW - Transcription, Genetic
UR - http://www.scopus.com/inward/record.url?scp=71449127067&partnerID=8YFLogxK
U2 - 10.1038/nsmb.1689
DO - 10.1038/nsmb.1689
M3 - Article
C2 - 19935686
SN - 1545-9993
VL - 16
SP - 1272
EP - 1278
JO - Nature Structural and Molecular Biology
JF - Nature Structural and Molecular Biology
IS - 12
ER -