Localization of three novel hybrid breakpoints and refinement of 18 marker assignments in the human 3cen-p21.1 region

Nai dy Wang; Joseph R. Testa; David I. Smith

doi:10.1016/S0888-7543(05)80110-7

Localization of three novel hybrid breakpoints and refinement of 18 marker assignments in the human 3cen-p21.1 region

Nai dy Wang, Joseph R. Testa, David I. Smith

Cancer Prevention and Control (CPC)

Wayne State University

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Using the human/hamster cell line UCTP2A-3, we have generated and isolated three hybrids, each containing a novel human chromosome 3p break. All chromosome 3 materials distal to the breaks were lost. Two of the breakpoints were produced using aphidicolin induction; the third breakpoint occurred spontaneously. The aphidicolin-induced breaks were localized to 3p21.1 in hybrid AR1 and to p14.1 in hybrid AR2. The spontaneous break was localized to 3p11 in hybrid 2A-3-1. These hybrids were used to sublocalize 18 chromosome 3 probes further to five regions within 3cen-p21.1. The new hybrids and sublocalized markers will be useful in the study and characterization of the 3p11, 3p14.1, and 3p21.1 segments of chromosome 3.

Original language	English
Pages (from-to)	891-896
Number of pages	6
Journal	Genomics
Volume	14
Issue number	4
DOIs	https://doi.org/10.1016/S0888-7543(05)80110-7
State	Published - Dec 1992

Keywords

Animals
Aphidicolin/toxicity
Base Sequence
Chromosome Banding
Chromosomes, Human, Pair 3
Cricetinae
DNA
Genetic Markers
Humans
Hybrid Cells
Karyotyping
Molecular Sequence Data
Polymerase Chain Reaction

Access to Document

10.1016/S0888-7543(05)80110-7

Cite this

@article{60e19ec69156407b9ec4b166bc668081,

title = "Localization of three novel hybrid breakpoints and refinement of 18 marker assignments in the human 3cen-p21.1 region",

abstract = "Using the human/hamster cell line UCTP2A-3, we have generated and isolated three hybrids, each containing a novel human chromosome 3p break. All chromosome 3 materials distal to the breaks were lost. Two of the breakpoints were produced using aphidicolin induction; the third breakpoint occurred spontaneously. The aphidicolin-induced breaks were localized to 3p21.1 in hybrid AR1 and to p14.1 in hybrid AR2. The spontaneous break was localized to 3p11 in hybrid 2A-3-1. These hybrids were used to sublocalize 18 chromosome 3 probes further to five regions within 3cen-p21.1. The new hybrids and sublocalized markers will be useful in the study and characterization of the 3p11, 3p14.1, and 3p21.1 segments of chromosome 3.",

keywords = "Animals, Aphidicolin/toxicity, Base Sequence, Chromosome Banding, Chromosomes, Human, Pair 3, Cricetinae, DNA, Genetic Markers, Humans, Hybrid Cells, Karyotyping, Molecular Sequence Data, Polymerase Chain Reaction",

author = "Wang, {Nai dy} and Testa, {Joseph R.} and Smith, {David I.}",

year = "1992",

month = dec,

doi = "10.1016/S0888-7543(05)80110-7",

language = "English",

volume = "14",

pages = "891--896",

journal = "Genomics",

issn = "0888-7543",

publisher = "Academic Press Inc.",

number = "4",

}

TY - JOUR

T1 - Localization of three novel hybrid breakpoints and refinement of 18 marker assignments in the human 3cen-p21.1 region

AU - Wang, Nai dy

AU - Testa, Joseph R.

AU - Smith, David I.

PY - 1992/12

Y1 - 1992/12

N2 - Using the human/hamster cell line UCTP2A-3, we have generated and isolated three hybrids, each containing a novel human chromosome 3p break. All chromosome 3 materials distal to the breaks were lost. Two of the breakpoints were produced using aphidicolin induction; the third breakpoint occurred spontaneously. The aphidicolin-induced breaks were localized to 3p21.1 in hybrid AR1 and to p14.1 in hybrid AR2. The spontaneous break was localized to 3p11 in hybrid 2A-3-1. These hybrids were used to sublocalize 18 chromosome 3 probes further to five regions within 3cen-p21.1. The new hybrids and sublocalized markers will be useful in the study and characterization of the 3p11, 3p14.1, and 3p21.1 segments of chromosome 3.

AB - Using the human/hamster cell line UCTP2A-3, we have generated and isolated three hybrids, each containing a novel human chromosome 3p break. All chromosome 3 materials distal to the breaks were lost. Two of the breakpoints were produced using aphidicolin induction; the third breakpoint occurred spontaneously. The aphidicolin-induced breaks were localized to 3p21.1 in hybrid AR1 and to p14.1 in hybrid AR2. The spontaneous break was localized to 3p11 in hybrid 2A-3-1. These hybrids were used to sublocalize 18 chromosome 3 probes further to five regions within 3cen-p21.1. The new hybrids and sublocalized markers will be useful in the study and characterization of the 3p11, 3p14.1, and 3p21.1 segments of chromosome 3.

KW - Animals

KW - Aphidicolin/toxicity

KW - Base Sequence

KW - Chromosome Banding

KW - Chromosomes, Human, Pair 3

KW - Cricetinae

KW - DNA

KW - Genetic Markers

KW - Humans

KW - Hybrid Cells

KW - Karyotyping

KW - Molecular Sequence Data

KW - Polymerase Chain Reaction

UR - http://www.scopus.com/inward/record.url?scp=0027104096&partnerID=8YFLogxK

UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=purepublist2023&SrcAuth=WosAPI&KeyUT=WOS:A1992KE78500010&DestLinkType=FullRecord&DestApp=WOS

U2 - 10.1016/S0888-7543(05)80110-7

DO - 10.1016/S0888-7543(05)80110-7

M3 - Article

C2 - 1478670

SN - 0888-7543

VL - 14

SP - 891

EP - 896

JO - Genomics

JF - Genomics

IS - 4

ER -

Localization of three novel hybrid breakpoints and refinement of 18 marker assignments in the human 3cen-p21.1 region

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this