Localization of complement-fixing cytotoxic monoclonal antibodies to subcutaneous tumors and liver metastases in a syngeneic immunocompetent rat colon carcinoma model

Kevin P. O'Boyle; M. Jules Mattes; Elin R. Sigurdson; Erica Sutanto-Ward; Chaitanya R. Divgi; Sydney Welt; Kathryn E. Wright

doi:10.1097/00002371-199404000-00003

Localization of complement-fixing cytotoxic monoclonal antibodies to subcutaneous tumors and liver metastases in a syngeneic immunocompetent rat colon carcinoma model

Kevin P. O'Boyle, M. Jules Mattes, Elin R. Sigurdson, Erica Sutanto-Ward, Chaitanya R. Divgi, Sydney Welt, Kathryn E. Wright

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

To study the immune effects of complement-fixing cytotoxic monoclonal antibodies (mAbs) in a syngeneic immunocompetent animal model, mouse mAbs reactive with the transplantable rat colon carcinoma K12/TRb were generated. This system was used in part because rats have a complement system superior to that of mice. Seven murine IgG mAbs that reacted strongly with cell surface determinants of the K12/TRb rat colon carcinoma cell line were produced by immunizing MRL/Mp-lpr/lpr autoimmune mice, known to produce an increased amount of complement-fixing IgG2a and IgG3 immune cytotoxic antibodies, with K12/TRb cells. These mAbs were screened for their specificity of reaction, and two of these mAbs were extensively tested for their ability to lyse cells in vitro and localize to K12/TRb tumors in syngeneic BDIX rats. IgG2a mAbs 27–3 and 61–5 were able to mediate both complement and lymphocyte cytotoxicity in vitro and localize to subcutaneous tumors and liver metastases in this immunocompetent rat model.

Original language	English
Pages (from-to)	175-184
Number of pages	10
Journal	Journal of Immunotherapy
Volume	15
Issue number	3
DOIs	https://doi.org/10.1097/00002371-199404000-00003
State	Published - Apr 1994

Keywords

Animals
Antibodies, Monoclonal/immunology
Antigens, Neoplasm/analysis
Colonic Neoplasms/immunology
Complement System Proteins/immunology
Cytotoxicity, Immunologic
Female
Immunocompetence
Liver Neoplasms, Experimental/immunology
Mice
Rats
Skin Neoplasms/immunology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1097/00002371-199404000-00003

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O'Boyle, K. P., Mattes, M. J., Sigurdson, E. R., Sutanto-Ward, E., Divgi, C. R., Welt, S., & Wright, K. E. (1994). Localization of complement-fixing cytotoxic monoclonal antibodies to subcutaneous tumors and liver metastases in a syngeneic immunocompetent rat colon carcinoma model. Journal of Immunotherapy, 15(3), 175-184. https://doi.org/10.1097/00002371-199404000-00003

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abstract = "To study the immune effects of complement-fixing cytotoxic monoclonal antibodies (mAbs) in a syngeneic immunocompetent animal model, mouse mAbs reactive with the transplantable rat colon carcinoma K12/TRb were generated. This system was used in part because rats have a complement system superior to that of mice. Seven murine IgG mAbs that reacted strongly with cell surface determinants of the K12/TRb rat colon carcinoma cell line were produced by immunizing MRL/Mp-lpr/lpr autoimmune mice, known to produce an increased amount of complement-fixing IgG2a and IgG3 immune cytotoxic antibodies, with K12/TRb cells. These mAbs were screened for their specificity of reaction, and two of these mAbs were extensively tested for their ability to lyse cells in vitro and localize to K12/TRb tumors in syngeneic BDIX rats. IgG2a mAbs 27–3 and 61–5 were able to mediate both complement and lymphocyte cytotoxicity in vitro and localize to subcutaneous tumors and liver metastases in this immunocompetent rat model.",

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AU - Sutanto-Ward, Erica

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Localization of complement-fixing cytotoxic monoclonal antibodies to subcutaneous tumors and liver metastases in a syngeneic immunocompetent rat colon carcinoma model

Abstract

Keywords

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