LIBS-based multi-element coded assay for ovarian cancer application

Y. Markushin, N. Melikechi, O. A. Marcano, S. Rock, E. Henderson, D. Connolly

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

13 Scopus citations

Abstract

We report on a new application of laser induced breakdown spectroscopy (LIBS) for the diagnosis of diseases such as ovarian cancer. We perform detection of ovarian cancer biomarker CA 125 based on LIBS measurements. Immunoconjugated Silicon particles are incubated with the affinity agarose beads carrying CA125 molecules. In the competitive affinity method Si particles carrying IgG molecules are pre-incubated with CA125. This pre-incubation decreases the numbers of free IgG molecules available for consequent interaction with the affinity beads. Thus less Si particles are attached to the agarose beads and consequently smaller Si peak area is measured by LIBS. We demonstrate a limit-ofdetection about 30 ppb for model protein avidin. We use two-element coded micro-particles to yield spectroscopic emission code using LIBS. We show that LIBS-based data collecting technique provides methodology for identification of biomarkers and cost-effective device for future clinical applications.

Original languageEnglish
Title of host publicationReporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications
DOIs
StatePublished - 2009
EventReporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications - San Jose, CA, United States
Duration: Jan 26 2009Jan 29 2009

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7190
ISSN (Print)1605-7422

Conference

ConferenceReporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications
Country/TerritoryUnited States
CitySan Jose, CA
Period01/26/0901/29/09

Keywords

  • Biomarker CA 125
  • Immunoassay
  • Laser-induced breakdown spectroscopy (LIBS)
  • Multielement coded assay
  • Ovarian cancer

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