Intravital imaging of metastatic behavior through a mammary imaging window

Dmitriy Kedrin; Bojana Gligorijevic; Jeffrey Wyckoff; Vladislav V. Verkhusha; John Condeelis; Jeffrey E. Segall; Jacco van Rheenen

doi:10.1038/nmeth.1269

Intravital imaging of metastatic behavior through a mammary imaging window

Dmitriy Kedrin, Bojana Gligorijevic, Jeffrey Wyckoff, Vladislav V. Verkhusha, John Condeelis, Jeffrey E. Segall, Jacco van Rheenen

Cancer Signaling and Microenvironment (CSM)

Research output: Contribution to journal › Article › peer-review

327 Scopus citations

Abstract

We report a technique to evaluate the same tumor microenvironment over multiple intravital imaging sessions in living mice. We optically marked individual tumor cells expressing photoswitchable proteins in an orthotopic mammary carcinoma and followed them for extended periods through a mammary imaging window. We found that two distinct microenvironments in the same orthotopic mammary tumor affected differently the invasion and intravasation of tumor cells.

Original language	English
Pages (from-to)	1019-1021
Number of pages	3
Journal	Nature Methods
Volume	5
Issue number	12
DOIs	https://doi.org/10.1038/nmeth.1269
State	Published - 2008

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10.1038/nmeth.1269

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AU - Condeelis, John

AU - Segall, Jeffrey E.

AU - van Rheenen, Jacco

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Intravital imaging of metastatic behavior through a mammary imaging window

Abstract

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