TY - JOUR
T1 - Intravesical Gene Therapy
T2 - In Vivo Gene Transfer Using Recombinant Vaccinia Virus Vectors
AU - Lee, Sharon S.
AU - Eisenlohr, Laurence C.
AU - McCue, Peter A.
AU - Mastrangelo, Michael J.
AU - Lattime, Edmund C.
PY - 1994/7/1
Y1 - 1994/7/1
N2 - Intratumoral gene transfer may be a significant tool in active immunotherapy. The ability to insert functional genes into a tumor in vitro and in vivo using recombinant vaccinia vectors was examined in the murine bladder tumor model. Vaccinia recombinants expressing the influenza hemagglutinin or nucleoprotein antigens infected/transfected murine (MB-49 and MBT-2) and human (T24) bladder tumor cell lines in vitro. Systemic vaccinia immunity was induced with as few as 10 plaque-forming units of recombinant vaccinia instilled intravesically, and the encoded protein was expressed in vivo in tumor and urothelium. However, preim-munity to vaccinia did not inhibit intravesical tumor transfection. Thus, recombinant vaccinia virus is effective in introducing foreign antigens locally into tumor in vivo, supporting its use in clinical immunotherapy.
AB - Intratumoral gene transfer may be a significant tool in active immunotherapy. The ability to insert functional genes into a tumor in vitro and in vivo using recombinant vaccinia vectors was examined in the murine bladder tumor model. Vaccinia recombinants expressing the influenza hemagglutinin or nucleoprotein antigens infected/transfected murine (MB-49 and MBT-2) and human (T24) bladder tumor cell lines in vitro. Systemic vaccinia immunity was induced with as few as 10 plaque-forming units of recombinant vaccinia instilled intravesically, and the encoded protein was expressed in vivo in tumor and urothelium. However, preim-munity to vaccinia did not inhibit intravesical tumor transfection. Thus, recombinant vaccinia virus is effective in introducing foreign antigens locally into tumor in vivo, supporting its use in clinical immunotherapy.
KW - Animals
KW - Carcinoma, Transitional Cell/genetics
KW - Female
KW - Genetic Vectors/genetics
KW - Mice
KW - Mice, Inbred C57BL
KW - Spleen/immunology
KW - T-Lymphocytes, Cytotoxic/immunology
KW - Transfection/methods
KW - Tumor Cells, Cultured
KW - Urinary Bladder Neoplasms/genetics
KW - Urinary Bladder/microbiology
KW - Vaccinia virus/genetics
UR - http://www.scopus.com/inward/record.url?scp=0028233204&partnerID=8YFLogxK
UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=purepublist2023&SrcAuth=WosAPI&KeyUT=WOS:A1994NU48800001&DestLinkType=FullRecord&DestApp=WOS
M3 - Article
C2 - 8012943
SN - 0008-5472
VL - 54
SP - 3325
EP - 3328
JO - Cancer Research
JF - Cancer Research
IS - 13
ER -
